Mouse Creatine kinase M-type(CKM) ELISA kit
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中文名稱：小鼠M型肌酸激酶(CKM)酶聯(lián)免疫試劑盒
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貨號：CSB-EL005459MO
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規(guī)格：96T/48T
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價格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠M型肌酸激酶(CKM)酶聯(lián)免疫試劑盒（CSB-EL005459MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的CKM含量。試劑盒檢測范圍為1.56 mU/mL-100 mU/mL，適用于小鼠模型研究中肌肉損傷評估、運動生理學實驗或藥物干預后能量代謝變化的分析，例如肌肉疾病模型機制研究、運動性肌損傷評價或心肌代謝相關(guān)藥物的科研篩選，為肌肉相關(guān)疾病的病理機制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Ckm ELISA Kit; Ckmm ELISA Kit; Creatine kinase M-type ELISA Kit; EC 2.7.3.2 ELISA Kit; Creatine kinase M chain ELISA Kit; Creatine phosphokinase M-type ELISA Kit; CPK-M ELISA Kit; M-CK ELISA Kit
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縮寫：CKM
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Uniprot No.：
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma
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檢測范圍：1.56 mU/mL-100 mU/mL
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靈敏度：0.39 mU/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Metabolism
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 86-95 1:2 Average % 98 Range % 92-100 1:4 Average % 90 Range % 87-96 1:8 Average % 91 Range % 85-95 -
回收率：
The recovery of mouse CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 89-99 EDTA plasma (n=4) 99 95-106 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
mU/ml OD1 OD2 Average Corrected 100 2.893 3.112 3.003 2.890 50 2.323 2.394 2.359 2.246 25 1.487 1.555 1.521 1.408 12.5 0.902 0.980 0.941 0.828 6.25 0.431 0.408 0.420 0.307 3.12 0.347 0.321 0.334 0.221 1.56 0.248 0.260 0.254 0.141 0 0.115 0.110 0.113 -
數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
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基因功能參考文獻：
- We conclude that ILK negatively and independently of PI3K regulated MEF2C phosphorylation activity and MCK mRNA expression in C2C12 cells PMID: 26788505
- The Fxn KO/Mck mice tested from one to two months of age showed abnormal gait patterns accompanied by a loss in motor skills. PMID: 25765157
- PI3K and AKT regulated skeletal muscle differentiation by regulating the expression of Myogenin and MCK. PMID: 23732671
- CK-M overexpression led to significantly increased contractile function at baseline and during adrenergic stimulation and increased survival after thoracic aortic constriction-induced heart failure. PMID: 22201686
- strong down-regulation of MCK activity contributes to F-actin instability and induces post-translational modification of alphaB-crystallin and desmin PMID: 21768101
- These studies indicate that the age-associated oxidative and nitrative modification of CKm results in a decrease in its activity and may cause structural changes that promote oligomerization and aggregation. PMID: 20195383
- Not involved in levels of creatine nnd creatine kinase in skeletal muscle of knockout mice. PMID: 12640020
- one of the consequences of M-CK and AK1 deficiency is hampered phosphoryl delivery to the actomyosin ATPase, resulting in a loss of contractile performance. PMID: 12730234
- Allosteric activation of phosphorylase b accounts for the accelerated glycogenolysis in CK-/- muscle during contraction. PMID: 12963789
- Data show that Six4 is transcriptional regulatory element X (TrexBF) in skeletal myocytes and transactivates the muscle creatine kinase enhancer PMID: 14966291
- the expression of muscle creatine kinase (MCK) gene in the heart and skeletal muscle of mice during aging PMID: 15040454
- metabolic failure induced by CK deficiency profoundly affects the ability of mice to engage in chronic bouts of endurance running exercise and that this decrease in performance is also associated with muscle wasting PMID: 15831533
- CK-MM autoantibodies can modulate the rate of CK clearance from the circulation in myositis PMID: 16810680
- Data show that spatial relations among organelles of muscle cells undergo adaptation in response to nonstructural stimuli such as metabolic (creatine kinase)deficiency. PMID: 16855221
- The creatine kinase energy transport system was evaluated in the failing mouse heart. PMID: 17481652
- Dec proteins repressed a MyoD-activated promoter activity of muscle creatine kinase gene through class A E-box in an HDAC1-independent manner. PMID: 17487425
- Creatine kinase (CK) and adenylate kinase (AK) are especially important in facilitating energy metabolism during very high energy demands. PMID: 19156695
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亞細胞定位：Cytoplasm.
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蛋白家族：ATP:guanido phosphotransferase family
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數(shù)據(jù)庫鏈接：
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