Mouse Complement fragment 4a,C4a ELISA Kit
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中文名稱：小鼠過敏毒素/補體片斷4a(C4a)酶聯(lián)免疫試劑盒
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貨號：CSB-E08517m
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規(guī)格：96T/48T
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價格：￥3200/￥2500
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其他：
產品詳情
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產品描述：小鼠過敏毒素/補體片斷4a(C4a)酶聯(lián)免疫試劑盒（CSB-E08517m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C4a含量。試劑盒檢測范圍為39 pg/mL-2500 pg/mL，適用于基礎免疫學研究，如炎癥性疾病動物模型中補體激活程度的動態(tài)監(jiān)測、藥物干預對補體系統(tǒng)影響的評估，或免疫細胞與補體互作機制等實驗場景。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
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縮寫：C4a
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：39 pg/mL-2500 pg/mL
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靈敏度：9.75 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Immunology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse C4a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1000 Average % 96 Range % 87-105 1:2000 Average % 90 Range % 84-100 1:4000 Average % 95 Range % 82-103 1:8000 Average % 96 Range % 84-103 -
回收率：
The recovery of mouse C4a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 87-105 EDTA plasma (n=4) 96 82-101 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 2500 2.786 2.687 2.737 2.599 1250 2.136 2.042 2.089 1.951 625 1.253 1.265 1.259 1.121 312 0.784 0.752 0.768 0.630 156 0.543 0.521 0.532 0.394 78 0.364 0.342 0.353 0.215 39 0.242 0.239 0.241 0.103 0 0.136 0.140 0.138 -
數(shù)據(jù)處理：
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貨期：3-5 working days
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