Mouse Complement factor I(CFI) ELISA kit

文獻引用 (1) 說明書下載

產品詳情

產品描述：

小鼠補體因子I(CFI)酶聯免疫試劑盒（CSB-EL005279MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CFI含量。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，適用于科研領域對補體系統相關機制的研究，如探究炎癥性疾病、免疫調控或感染過程中CFI的表達變化?？捎糜诩毎Ｐ?、動物實驗或生物標志物篩選等基礎研究場景，助力補體系統功能解析及免疫相關疾病機制的深入探索。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Cfi ELISA Kit; IfComplement factor I ELISA Kit; EC 3.4.21.45 ELISA Kit; C3B/C4B inactivator) [Cleaved into: Complement factor I heavy chain; Complement factor I light chain] ELISA Kit
縮寫：

CFI
Uniprot No.：

Q61129
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CFI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	97
	Range %	93-100
1:2000	Average %	85
	Range %	81-89
1:4000	Average %	96
	Range %	93-99
1:8000	Average %	87
	Range %	85-90

回收率：

The recovery of mouse CFI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-106
EDTA plasma (n=4)	85	81-89

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins. Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces. The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization.
基因功能參考文獻：
1. Factor I-mediated generation of activated C3 fragments in the circulation is a critical determinant for the development of MPGN2 associated with factor H deficiency. PMID: 18202746
亞細胞定位：

Secreted, extracellular space.
蛋白家族：

Peptidase S1 family
組織特異性：

Expressed in the liver by hepatocytes. Also present in other cells such as monocytes, fibroblasts or keratinocytes.
數據庫鏈接：

KEGG: mmu:12630

STRING: 10090.ENSMUSP00000077074

UniGene: Mm.117180