Mouse Complement 4,C4 ELISA Kit

Mouse Complement 4,C4 ELISA Kit

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中文名稱：

小鼠補體蛋白4(C4)酶聯(lián)免疫試劑盒
貨號：

CSB-E08707m
規(guī)格：

96T/48T
價格：

￥3200/￥2500
促銷：
其他：

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產(chǎn)品描述：

小鼠補體蛋白4(C4)酶聯(lián)免疫試劑盒（CSB-E08707m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C4B含量。試劑盒檢測范圍為0.156 μg/mL-10 μg/mL，可應(yīng)用于小鼠模型中的補體系統(tǒng)功能研究，例如自身免疫性疾病（如紅斑狼瘡、類風(fēng)濕關(guān)節(jié)炎）、炎癥反應(yīng)機制探索或感染性疾病進程中C4蛋白的動態(tài)變化分析，亦可用于藥物開發(fā)中補體相關(guān)通路調(diào)控效應(yīng)的科研評估。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

C4b ELISA kit; C4Complement C4-B [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain] ELISA kit
縮寫：

C4B
Uniprot No.：

P01029
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 μg/mL-10 μg/mL
靈敏度：

0.039 μg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-95
1:200	Average %	102
	Range %	97-107
1:400	Average %	91
	Range %	85-97
1:800	Average %	97
	Range %	91-103

回收率：

The recovery of mouse C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/m	OD1	OD2	Average	Corrected
10	2.605	2.648	2.627	2.463
5	2.312	2.335	2.324	2.160
2.5	1.857	1.867	1.862	1.698
1.25	1.136	1.103	1.120	0.956
0.625	0.798	0.785	0.792	0.628
0.312	0.435	0.412	0.424	0.260
0.156	0.316	0.304	0.310	0.146
0	0.163	0.165	0.164

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. Catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.
基因功能參考文獻：
1. These data show that Borrelia burgdorferi OspC inhibits the classical and lectin complement pathways and competes with complement protein C2 for C4b binding. PMID: 28873507
2. C4 mediated synapse elimination during postnatal development PMID: 26814963
3. role in suppressing autoantibody production elicited by S. pneumoniae antigens PMID: 25339671
4. a deficiency in C4b does not alter radiation-induced lung disease in the C57BL/6 mouse model PMID: 23259761
5. C4 modulates interstitial inflammation in experimental glomerulonephritis. PMID: 11726230
6. Complement C4 provides an important protective role against the development of systemic lupus erythematosus. PMID: 11801636
7. identified the murine follicular dendritic cell marker, FDC-M2, as complement c4. FDC-M2 was detectable at sites of immune complex-mediated inflammatory disease PMID: 12115608
8. C4 normally helps prevent early stages of autoimmune disease and that C4 deficiency predisposes to abnormal regulation of autoreactive B cells. PMID: 12207352
9. Macrophages derived from bone marrow produce sufficient C4 protein to restore the humoral response to NP-KLH hapten in C4-deficient animals when administered along with thymus-dependent antigen. PMID: 12421924
10. effects of complement depletion and deficiencies of C4, C5, and complement receptors 1 and 2 on mouse survival after intravenous exposure to S aureus PMID: 15192652
11. We found that deletion of the C4 gene does not significantly change either the time of onset or the severity and tempo of myelin oligodendrocyte-induced EAE compared with controls with a fully intact complement system. PMID: 15390104
12. In comparison with C57BL/6 mice, MRL/1pr mice had depressed C4 levels as early as 3 weeks of age. PMID: 17971229
13. The increased mortality of C4(-/-) or Factor B(-/-) mice indicates that these two pathways of complement activation are required for survival in mousepox. PMID: 19112490
14. Increased transcription of the complement C4 gene in the Rag1(-/-) intestine. PMID: 19200601
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亞細胞定位：

Secreted. Cell junction, synapse. Cell projection, axon. Cell projection, dendrite.
數(shù)據(jù)庫鏈接：

KEGG: mmu:12268

STRING: 10090.ENSMUSP00000069418

UniGene: Mm.439678

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.