Mouse Calcitonin,CT ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠降鈣素(CT)酶聯免疫試劑盒（CSB-E05133m）檢測血清、血漿、組織培養上清液、組織勻漿樣本中的CALCA含量。CALCA 是重要靶點，它編碼降鈣素基因相關肽等，在痛覺傳導、血管調節等生理過程起關鍵作用。研究機制上，聚焦其信號通路及受體相互作用，旨在通過藥物干預該靶點，為偏頭痛、心血管病等疾病治療提供新思路。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，本產品專為科研實驗設計，可廣泛應用于小鼠疾病模型構建、細胞因子調控機制研究、藥物干預效果評估等基礎研究領域，為代謝性疾病及骨骼系統研究提供可靠檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CALCA Calcitonin
縮寫：

CALCA
Uniprot No.：

P70160
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
Range %	80-88
1:2	Average %	95
Range %	91-99
1:4	Average %	86
Range %	83-89
1:8	Average %	97
Range %	92-102

回收率：

The recovery of mouse CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-93
EDTA plasma (n=4)	100	95-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
亞細胞定位：

Secreted.
蛋白家族：

Calcitonin family
數據庫鏈接：

STRING: 10090.ENSMUSP00000032907

UniGene: Mm.4361