Mouse C-Peptide ELISA Kit
產(chǎn)品詳情
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產(chǎn)品描述：
小鼠C肽(C-Peptide)酶聯(lián)免疫試劑盒（CSB-E05068m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的C-Peptide含量。C-Peptide即C肽，是胰島素原裂解產(chǎn)生的與胰島素等分子的肽類物質(zhì)。研究發(fā)現(xiàn)它有獨立于胰島素的生物學(xué)作用，可改善神經(jīng)、腎臟等器官的血流，調(diào)節(jié)細胞內(nèi)信號傳導(dǎo)，其機制為結(jié)合特定細胞膜受體，激活下游信號通路，正被深入研究以挖掘更多臨床價值。試劑盒檢測范圍為2.86 ng/mL-50 ng/mL，糖尿病模型研究、胰島素分泌機制探索及代謝調(diào)控實驗、胰島素相關(guān)代謝研究、糖尿病動物模型評估以及藥物干預(yù)效果分析、科研領(lǐng)域?qū)Ζ录毎δ軇討B(tài)監(jiān)測本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：
C-Peptide
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種屬：
Mus musculus (Mouse)
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樣本類型：
serum, plasma
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檢測范圍：
3.125 ng/ml-50 ng/ml
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靈敏度：
0.625 ng/ml
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反應(yīng)時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領(lǐng)域：
Metabolism
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
85-99 |
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| 1:2 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:4 |
Average % |
95 |
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| Range % |
87-106 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-100 |
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回收率：
| The recovery of mouse C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
89-100 |
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| EDTA plasma (n=4) |
95 |
91-103 |
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本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse C-peptide antibody.
- Six vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial HRP-conjugated C-peptide antibody(6 ml/bottle) ---Bind to the C-peptide in the samples or standards and react with the substrate to make the solution chromogenic.
- One vial Wash Buffer (20x concentrate) (15ml/bottle) ---Wash away unbound or free substances.
- Substrate A (1 x 7 ml)
- Substrate B (1 x 7 ml)
- One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
引用文獻
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Ergosterol Ameliorates Diabetic Nephropathy by Attenuating Mesangial Cell Proliferation and Extracellular Matrix Deposition via the TGF-β1/Smad2 Signaling Pathway
Dong Z, et al,Nutrients,2019
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Loss of complex O-glycosylation impairs exocrine pancreatic function and induces MODY8-like diabetes in mice
Wolters-Eisfeld G.et al,experimental and molecular medicine,2018
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DUSP1 recuses diabetic nephropathy via repressing JNK-Mff-mitochondrial fission pathways
Junqin Sheng.et al,Journal of cellular physiology,2018
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Cyanidin-3-O-glucoside ameliorates diabetic nephropathy through regulation of glutathione pool
YanQin.et al,Biomedicine & Pharmacotherapy,2018
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SIRT3 Facilitates Amniotic Fluid Stem Cells to Repair Diabetic Nephropathy Through Protecting Mitochondrial Homeostasis by Modulation of Mitophagy
Feng J.et al,Cellular Physiology and Biochemistry,2018
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HIF1α deletion facilitates adipose stem cells to repair renal fibrosis in diabetic mice
Tang Q.et al,In Vitro Cell Dev Biol Anim,2018
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6-Shogaol ameliorates diabetic nephropathy through anti-inflammatory, hyperlipidemic, anti-oxidative activity in db/db mice
Xu Y.et al,Biomed Pharmacother,2017
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Combinatorial Treatment of Bone Marrow Transplantation and Regulatory T Cells Improves Glycemia in Streptozotocin-diabetic Mice.
Min H.et al,Exp Clin Endocrinol Diabetes.,2015
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Homoarginine supplementation improves blood glucose in diet-induced obese mice
Stockebrand M. et al,Amino Acids. ,2015
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