Mouse Apolipoprotein C-II(APOC2) ELISA kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠載脂蛋白C-II(APOC2)酶聯免疫試劑盒（CSB-EL001932MO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOC2含量。APOC2即載脂蛋白C2，它在脂質代謝中發揮重要作用，是脂蛋白脂肪酶（LPL）的激活劑，可促進乳糜微粒和極低密度脂蛋白的分解代謝。目前關于APOC2的研究，多圍繞其在脂質代謝異常相關疾病如高脂血癥等中的機制，探索其作為治療靶點的潛力。試劑盒檢測范圍為18.8 ng/mL-1200 ng/mL，科研人員可通過研究脂代謝異常相關疾病（如肥胖、糖尿病）的分子機制，或評估藥物干預對脂代謝途徑的影響；為心血管疾病及代謝綜合征的基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Apoc2Apolipoprotein C-II ELISA kit; Apo-CII ELISA kit; ApoC-II ELISA kit; Apolipoprotein C2 ELISA kit
縮寫：

APOC2
Uniprot No.：

Q05020
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

18.8 ng/mL-1200 ng/mL
靈敏度：

9.4 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	97
	Range %	88-102
1:40	Average %	93
	Range %	85-98
1:80	Average %	103
	Range %	96-107
1:160	Average %	104
	Range %	95-109

回收率：

The recovery of mouse APOC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	101	94-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase.
亞細胞定位：

Secreted.
蛋白家族：

Apolipoprotein C2 family
組織特異性：

Adult and fetal liver, intestine and peritoneal macrophages.
數據庫鏈接：

KEGG: mmu:105886299

STRING: 10090.ENSMUSP00000003074

UniGene: Mm.28394