Mouse Angiopoietin-related protein 7(ANGPTL7) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠血管生成素相關(guān)蛋白7(ANGPTL7)酶聯(lián)免疫試劑盒（CSB-EL001715MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ANGPTL7含量。ANGPTL7是一個靶點。其背景上，它可能與某些生理或病理過程相關(guān)。在研究機(jī)制方面，目前可能著重探索其在疾病發(fā)生發(fā)展中的作用，比如對血管生成、細(xì)胞增殖等的影響，后續(xù)研究或為相關(guān)疾病治療提供新的方向和策略。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，本試劑盒適用于小鼠模型中ANGPTL7蛋白水平的定量分析，可應(yīng)用于心血管疾病、代謝調(diào)控或組織纖維化等領(lǐng)域的機(jī)制研究，例如在基因編輯模型或藥物干預(yù)實驗中評估ANGPTL7的動態(tài)變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Angptl7Angiopoietin-related protein 7 ELISA kit; Angiopoietin-like protein 7 ELISA kit
縮寫：

ANGPTL7
Uniprot No.：

Q8R1Q3
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-94
1:2	Average %	98
	Range %	93-103
1:4	Average %	102
	Range %	95-109
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of mouse ANGPTL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-101
EDTA plasma (n=4)	93	88-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Has a role in the formation and organization of the extracellular matrix. In the eye, it functions as a mediator of dexamethasone-induced matrix deposition in the trabecular meshwork, the tissue responsible for the outflow of the ocular aqueous humor and for the maintenance of intraocular pressure. Is a negative regulator of angiogenesis in the cornea, and plays a major role in maintaining corneal avascularity and transparency.
基因功能參考文獻(xiàn)：
1. Angptl7 is a factor that promotes pro-inflammatory responses in macrophages through the P38 MAPK signaling pathway PMID: 26973239
2. Angptl7 was indispensable for bone marrow microenvironment to support hematopoietic stem cells repopulation PMID: 25652910
亞細(xì)胞定位：

Secreted.
數(shù)據(jù)庫鏈接：

KEGG: mmu:654812

STRING: 10090.ENSMUSP00000030840

UniGene: Mm.388929