Mouse Alpha-fetoprotein,AFP ELISA Kit

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中文名稱：

小鼠甲種胎兒球蛋白/甲胎蛋白(AFP)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08282m
規(guī)格：

96T/48T
價(jià)格：

￥3800/￥2500
促銷：
其他：

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小鼠甲種胎兒球蛋白/甲胎蛋白(AFP)酶聯(lián)免疫試劑盒（CSB-E08282m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿.樣本中的AFP含量。AFP即甲胎蛋白，是一種糖蛋白。其正常情況下主要由胎兒肝細(xì)胞及卵黃囊合成。在成人中，AFP升高常見(jiàn)于肝癌等惡性腫瘤。研究上常將其作為腫瘤標(biāo)志物，通過(guò)檢測(cè)其水平輔助肝癌等疾病的診斷、監(jiān)測(cè)及預(yù)后評(píng)估。試劑盒檢測(cè)范圍為1.23 ng/ml-900 ng/ml.，靈敏度為1.23 ng/ml.。科研人員可通過(guò)探究肝損傷修復(fù)、腫瘤發(fā)生發(fā)展或胚胎發(fā)育相關(guān)機(jī)制，為構(gòu)建疾病模型、評(píng)估干預(yù)效果提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
別名：

AfpAlpha-fetoprotein ELISA kit; Alpha-1-fetoprotein ELISA kit; Alpha-fetoglobulin ELISA kit
縮寫：

AFP
Uniprot No.：

P02772
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates.
檢測(cè)范圍：

1.23 ng/ml-900 ng/ml.
靈敏度：

1.23 ng/ml.
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	34.056	34.846	35.720	33.819	34.253	34.648
SD	0.035	0.038	0.042	0.036	0.042	0.053
CV(%)	4.481	4.744	5.103	4.645	5.344	6.658

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

82-93

1:2

Average %

Range %

94-104

1:4

Average %

Range %

96-108

1:8

Average %

Range %

80-100

回收率：

The recovery of mouse AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	83-97
EDTA plasma (n=4)	96	92-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
900	2.379	2.252	2.316	2.161
300	1.975	1.876	1.926	1.771
100	1.305	1.304	1.305	1.150
33.33	0.812	0.779	0.796	0.641
11.11	0.534	0.494	0.514	0.359
3.7	0.372	0.349	0.361	0.206
1.23	0.264	0.249	0.257	0.102
0	0.161	0.149	0.155

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Re-evaluation of the anticarcinogenic effect of metformin in a chemically-induced hepatocellular carcinoma model not associated with diabetes F AM El-Deeb,Egyptian Journal of Basic and Applied Sciences,2022
Possible ameliorating effect of isoquercetin on diethylnitrosamine-induced hepatocellular carcinoma in albino mice Kholoud A. Omran,Egyptian Journal of Basic and Applied Sciences,2022
Spirulina inhibits hepatocellular carcinoma through activating p53 and apoptosis and suppressing oxidative stress and angiogenesis Yomna I Mahmoud,Life Sciences,2020
Effect of protocatechuic acid-layered double hydroxide nanoparticles on diethylnitrosamine/phenobarbital-induced hepatocellular carcinoma in mice Gani SA,PLoS One,2019
Screening of biomarkers for liver adenoma in low-dose-rate gamma-ray-irradiated mice. Sugihara T.et al,Int J Radiat Biol,2018

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功能：

Binds estrogens, fatty acids and metals.
基因功能參考文獻(xiàn)：
1. endogenous AFP expression in the perinatal liver is reduced in the absence of beta-catenin, suggesting that beta-catenin regulates AFP during liver development. PMID: 22544812
2. Findings indicate that AFP expressed under experimental cryptorchid conditions plays a role as a regulatory factor in spermatogenesis and in hepatic generation. PMID: 21573244
3. AFP mRNA and VEGF-C mRNA in peripheral blood were significantly correlated with recurrence and metastasis of hepatocellular carcinoma. PMID: 20506647
4. A DEHP treatment-induced phenotype was identified with lack of glycogen accumula-tion and intracytoplasmic localization of beta-catenin which was associated with increased AFP gene expression. PMID: 20307648
5. The deficits observed in parental behavior of AFP knockout females could not be explained by any changes in olfactory function, novelty recognition or anxiety PMID: 20109458
6. Foxa1 acts as a pioneer transcription factor in de novo activation of Afp, by exploiting a lack of methylation at juxtaposed transposed elements PMID: 20348100
7. The level of AFP mRNA in peripheral blood may indicate recurrence and/or metastasis after curative resection of hepatocellular carcinoma. PMID: 12113675
8. AFP affects cell proliferation by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 genes PMID: 12118941
9. A murine Nkx2.8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor. PMID: 12167706
10. The mouse alpha-fetoprotein promoter is repressed in HepG2 hepatoma cells by hepatocyte nuclear factor-3 PMID: 12215259
11. AFP has a role in female fertility, but not in male fertility or survival, in mice PMID: 12297623
12. mouse and human alpha-fetoprotein enhancers are strikingly different PMID: 15028291
13. AFP protects the developing female brain from the adverse effects of prenatal estrogen exposure and clarify a long-running debate on the role of this fetal protein in brain sexual differentiation PMID: 16479017
14. 30-kDa protein is a novel isoform of AUF1 family and is the main component of the DAP-II complex that binds to the DAS sequence PMID: 16514630
15. The sex difference in the ability to show preovulatory LH surges depends on the prenatal actions of estrogens in the male hypothalamus, is lost in Afp(-/-) females. PMID: 18202134
16. data indicate that this enhancer region is required for alpha-fetoprotein and albumin activation early in liver development and alpha-fetoprotein reactivation during liver regeneration PMID: 19782060
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亞細(xì)胞定位：

Secreted.
蛋白家族：

ALB/AFP/VDB family
組織特異性：

Plasma.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:11576

STRING: 10090.ENSMUSP00000041006

UniGene: Mm.358570

Mouse Alpha-fetoprotein,AFP ELISA Kit

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