Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

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中文名稱：

小鼠丙氨酸轉氨酶/谷丙轉氨酶(ALT/GPT)酶聯免疫試劑盒
貨號：

CSB-E16539m
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠丙氨酸轉氨酶/谷丙轉氨酶(ALT/GPT)酶聯免疫試劑盒（CSB-E16539m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、細胞裂解物樣本中的ALT/GPT含量。ALT/GPT即谷丙轉氨酶，主要存在于肝細胞內。肝細胞受損時其釋放入血，致血清ALT/GPT水平升高，是反映肝細胞損傷的敏感指標。臨床常通過檢測其活性輔助診斷肝臟疾病，如肝炎、肝硬化等，研究也圍繞其在疾病中的變化機制展開。試劑盒檢測范圍為0.156 mU/mL-10 mU/mL，廣泛應用于肝損傷模型評估、藥物肝毒性研究、代謝性疾病機制探索等科研領域，特別適用于體外培養肝細胞或動物模型中肝臟相關病理生理變化的動態監測，為肝臟功能研究提供可靠的實驗工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Gpt ELISA kit; Gpt1Alanine aminotransferase 1 ELISA kit; ALT1 ELISA kit; EC 2.6.1.2 ELISA kit; Glutamate pyruvate transaminase 1 ELISA kit; GPT 1 ELISA kit; Glutamic--alanine transaminase 1 ELISA kit; Glutamic--pyruvic transaminase 1 ELISA kit
縮寫：

ALT/GPT
Uniprot No.：

Q8QZR5
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, cell lysates
檢測范圍：

0.156 mU/mL-10 mU/mL
靈敏度：

0.039 mU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-95
1:2	Average %	97
	Range %	92-101
1:4	Average %	106
	Range %	98-113
1:8	Average %	94
	Range %	87-97

回收率：

The recovery of mouse ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	102	95-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
10	2.645	2.566	2.606	2.421
5	2.015	1.855	1.935	1.750
2.5	1.194	1.155	1.175	0.990
1.25	0.777	0.796	0.787	0.602
0.625	0.492	0.461	0.477	0.292
0.312	0.335	0.344	0.340	0.155
0.156	0.278	0.288	0.283	0.098
0	0.191	0.178	0.185

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
基因功能參考文獻：
1. Dexamethasone induces a metabolic adaptive ALT isoenzyme response in livers of C57/BL6 mice. PMID: 22609950
2. PKCdelta activation and protein levels are elevated in an animal model of steatohepatitis, which was recapitulated in a cell model, supporting the conclusion that PKCdelta plays a role in ALT release, the ER stress signal, and cell death PMID: 20971848
3. In experiments where mice were provided ethanol chronically in drinking water, where stress is minimal, higher levels of ALT were noted in animals in the ethanol group as compared with animals in the control group. PMID: 15652761
亞細胞定位：

Cytoplasm.
蛋白家族：

Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
組織特異性：

Mainly expressed in liver, intestine, colon and white adipose tissue.
數據庫鏈接：

KEGG: mmu:76282

STRING: 10090.ENSMUSP00000023203

UniGene: Mm.30130

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.