Mouse 5-Hydroxytryptamine,5HT ELISA Kit

Mouse 5-Hydroxytryptamine,5HT ELISA Kit

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文獻引用 (18) 評價 (2) 說明書下載

中文名稱：

小鼠5羥色胺(5-HT)酶聯免疫試劑盒
貨號：

CSB-E08365m
規格：

96T/48T
價格：

￥3800/￥2500
促銷：
其他：

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產品描述：

小鼠5羥色胺(5-HT)酶聯免疫試劑盒（CSB-E08365m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的5HT含量。5-HT即血清素，是一種重要的神經遞質。它廣泛分布于中樞神經系統與外周組織。在研究機制上，參與調節情緒、睡眠、食欲等多種生理功能，異常水平與抑郁癥等精神疾病相關。當前圍繞其開展的研究，旨在開發相關藥物來治療各類身心疾病。試劑盒檢測范圍為250 pg/mL-4000 pg/ml，該產品適用于神經科學研究中的遞質動態分析、藥理學實驗的干預效果評估、行為學模型驗證以及代謝相關疾病動物模型的機制探索本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

5HT
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

250 pg/mL-4000 pg/mL
靈敏度：

125 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse 5HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	90-89
1:200	Average %	90
	Range %	86-94
1:400	Average %	84
	Range %	80-88
1:800	Average %	97
	Range %	92-101

回收率：

The recovery of mouse 5HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-99
EDTA plasma (n=4)	89	83-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
4000	0.130	0.120	0.125
2000	0.243	0.241	0.242
1000	0.452	0.419	0.436
500	0.792	0.831	0.812
250	1.068	1.120	1.094
0	2.151	2.026	2.089

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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Quercetin Alleviates Breast Cancer-Related Depression by Inhibiting Neutrophil Extracellular Traps via Inhibition of Sphingosine 1-Phosphate/Sphingosine 1-Phosphate Receptor Axis Q Zhu, Y Tao, Y Han, Y He, Y Fu, H Yang,Phytotherapy Research,2025
Inhaling arsenic aggravates airway hyperreactivity by upregulating PNEC-sourced 5-HT in OVA-induced allergic asthma X Lu, ZX Tan, YX Yao, ZY Li, YY Zhu, QQ Yang,Ecotoxicology and Environmental Safety,2025
Impact of Altered Gut Microbiota on Ketamine-Induced Conditioned Place Preference in Mice C Li, C Zhu, G Tu, Z Chen, Z Mo,Neuropsychiatric Disease and Treatment,2024
The role of gut microbiota in anorexia induced by T-2 toxin T Huang,Ecotoxicology and environmental safety,2024
Tryptophan metabolic pathway plays a key role in the stress-induced emotional eating Y Zhu,Current research in food science,2024
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Asperosaponin VI Protects Against Bone Loss Due to Hindlimb Unloading in Skeletally Growing Mice Through Regulating Microbial Dysbiosis Altering the 5-HT Pathway YB Niu,Calcified tissue international,2023
Theabrownin inhibits obesity and non-alcoholic fatty liver disease in mice via serotonin-related signaling pathways and gut-liver axis HY Li,Journal of advanced research,2023
Anti-depressant-like effect of fermented Gastrodia elata Bl. by regulating monoamine levels and BDNF/NMDAR pathways in mice M Gao,Journal of ethnopharmacology,2022
Bisphenol A impairs cognitive function and 5-HT metabolism in adult male mice by modulating the microbiota-gut-brain axis Yinhua Ni,Chemosphere,2021
Saponin extracts from Gynostemma pentaphyllum (Thunb.) Makino display sedative-hypnotic and anxiolytic effects CY Shen,Industrial Crops and Products,2020
Titanium dioxide nanoparticles via oral exposure leads to adverse disturbance of gut microecology and locomotor activity in adult mice Zhang S,Archives of Toxicology,2020
Modulation of TGF-β/Smad and ERK signaling pathways mediates the anti-fibrotic effect of mirtazapine in mice El-Tanbouly DM.et al,Toxicol Appl Pharmacol,2017
Physiological and behavioral responses in offspring mice following maternal exposure to sulfamonomethoxine during pregnancy. Zhang Q.et al,Neurosci Lett.,2016

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平均分:

4.5分 - 2 個評價

樣品類型：細胞培養上清液

樣品信息：小鼠

稀釋比：其他 1:2

產品評價：我用檢測PC12細胞上清液，標準曲線的OD值為y = -1.0317x + 2.6063，R2 = 0.9866 樣本的OD值為0.1084~0.2402 快速便捷，非常好用

By 廖老師

樣品類型：其他樣品處理方法細胞勻漿液，用適量ripa裂解后取離心后上清做

樣品信息：小鼠

稀釋比：其他 1:20

產品評價： CSB-E08365m檢測不同濃度的戊酸給給藥后的LLC培養上清液以及細胞勻漿液的5HT含量，標準曲線的OD值為：4000-0.08，2000-0.15，1000-0.17，500-0.43，250-0.87，0-1.01，樣品OD值為0.19~0.67，陰性對照是0.99左右，產品快速便捷，操作簡單，重復性較好，但是貌似沒有對細胞樣品的處理指南。陰性對照是水（0.9900，0.9142，0.9521），樣品則為0.19~0.67；標曲的0濃度吸光值為（1.0943，1.0101）。

By 廖老師

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.