Mouse 5-Hydroxytryptamine,5HT ELISA Kit

Mouse 5-Hydroxytryptamine,5HT ELISA Kit

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中文名稱：

小鼠5羥色胺(5-HT)酶聯(lián)免疫試劑盒
貨號：

CSB-E08365m
規(guī)格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

小鼠5羥色胺(5-HT)酶聯(lián)免疫試劑盒（CSB-E08365m）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的5HT含量。5-HT即血清素，是一種重要的神經(jīng)遞質。它廣泛分布于中樞神經(jīng)系統(tǒng)與外周組織。在研究機制上，參與調節(jié)情緒、睡眠、食欲等多種生理功能，異常水平與抑郁癥等精神疾病相關。當前圍繞其開展的研究，旨在開發(fā)相關藥物來治療各類身心疾病。試劑盒檢測范圍為250 pg/mL-4000 pg/ml，該產(chǎn)品適用于神經(jīng)科學研究中的遞質動態(tài)分析、藥理學實驗的干預效果評估、行為學模型驗證以及代謝相關疾病動物模型的機制探索本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

5HT
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

250 pg/mL-4000 pg/mL
靈敏度：

125 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse 5HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	90-89
1:200	Average %	90
	Range %	86-94
1:400	Average %	84
	Range %	80-88
1:800	Average %	97
	Range %	92-101

回收率：

The recovery of mouse 5HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-99
EDTA plasma (n=4)	89	83-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
4000	0.130	0.120	0.125
2000	0.243	0.241	0.242
1000	0.452	0.419	0.436
500	0.792	0.831	0.812
250	1.068	1.120	1.094
0	2.151	2.026	2.089

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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Inhaling arsenic aggravates airway hyperreactivity by upregulating PNEC-sourced 5-HT in OVA-induced allergic asthma X Lu, ZX Tan, YX Yao, ZY Li, YY Zhu, QQ Yang,Ecotoxicology and Environmental Safety,2025
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The role of gut microbiota in anorexia induced by T-2 toxin T Huang,Ecotoxicology and environmental safety,2024
Tryptophan metabolic pathway plays a key role in the stress-induced emotional eating Y Zhu,Current research in food science,2024
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Asperosaponin VI Protects Against Bone Loss Due to Hindlimb Unloading in Skeletally Growing Mice Through Regulating Microbial Dysbiosis Altering the 5-HT Pathway YB Niu,Calcified tissue international,2023
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Bisphenol A impairs cognitive function and 5-HT metabolism in adult male mice by modulating the microbiota-gut-brain axis Yinhua Ni,Chemosphere,2021
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Modulation of TGF-β/Smad and ERK signaling pathways mediates the anti-fibrotic effect of mirtazapine in mice El-Tanbouly DM.et al,Toxicol Appl Pharmacol,2017
Physiological and behavioral responses in offspring mice following maternal exposure to sulfamonomethoxine during pregnancy. Zhang Q.et al,Neurosci Lett.,2016

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平均分:

4.5分 - 2 個評價

樣品類型：細胞培養(yǎng)上清液

樣品信息：小鼠

稀釋比：其他 1:2

產(chǎn)品評價：我用檢測PC12細胞上清液，標準曲線的OD值為y = -1.0317x + 2.6063，R2 = 0.9866 樣本的OD值為0.1084~0.2402 快速便捷，非常好用

By 廖老師

樣品類型：其他樣品處理方法細胞勻漿液，用適量ripa裂解后取離心后上清做

樣品信息：小鼠

稀釋比：其他 1:20

產(chǎn)品評價： CSB-E08365m檢測不同濃度的戊酸給給藥后的LLC培養(yǎng)上清液以及細胞勻漿液的5HT含量，標準曲線的OD值為：4000-0.08，2000-0.15，1000-0.17，500-0.43，250-0.87，0-1.01，樣品OD值為0.19~0.67，陰性對照是0.99左右，產(chǎn)品快速便捷，操作簡單，重復性較好，但是貌似沒有對細胞樣品的處理指南。陰性對照是水（0.9900，0.9142，0.9521），樣品則為0.19~0.67；標曲的0濃度吸光值為（1.0943，1.0101）。

By 廖老師

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.