Monkey Tumor necrosis factor α,TNF-α ELISA KIT

Monkey Tumor necrosis factor α,TNF-α ELISA KIT

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中文名稱：

猴腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒
貨號：

CSB-E10039Mo
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

猴腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒（CSB-E10039Mo）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNF含量。TNF即腫瘤壞死因子，是一種多功能細胞因子。在背景上，它最初因能使腫瘤組織出血壞死而被發現。其研究機制主要涉及參與免疫調節、炎癥反應等過程。異常的TNF表達與多種疾病相關，如類風濕關節炎等，是相關疾病治療的重要靶點。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，該靶標常用于靈長類動物感染性疾病、自身免疫模型及炎癥機制研究；適用于恒河猴、食蟹猴等非人靈長類動物的基礎研究，如病毒感染模型中細胞因子風暴評估、免疫抑制劑作用機制探索，或通過組織樣本分析特定器官炎癥微環境變化，為神經退行性疾病、代謝綜合征等科研方向提供關鍵數據支持；適用于實驗室常規蛋白定量分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
縮寫：

TNF
Uniprot No.：

P48094
種屬：

Monkey
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.105	2.097	2.101	2.008
50	1.863	1.805	1.834	1.741
25	1.412	1.395	1.404	1.311
12.5	0.909	0.896	0.903	0.810
6.25	0.594	0.549	0.572	0.479
3.12	0.306	0.298	0.302	0.209
1.56	0.191	0.187	0.189	0.096
0	0.096	0.090	0.093

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

平均分:

5.0分 - 1 個評價

樣品類型：細胞培養上清液

樣品信息：其他猴

稀釋比：其他 1：3，1;5，1:10

產品評價：我用CSB-E10039Mo檢測食蟹猴細胞培養上清液，標曲OD值范圍為3.1274-0.2045，樣品檢測OD值范圍為1.112-0.109，檢測良好，標曲正常。

By 榮老師

功能：

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
基因功能參考文獻：
1. These interactions result in the induction of the TNF signaling pathway, activation of apoptosis, and DNA-damage stress response. PMID: 24743303
2. Findings showed that both mucosal compartments harbor similar percentages of memory CD4(+) T cells and displayed comparable cytokine TNF-alpha responses to mitogenic stimulations prior to infection. PMID: 24610016
3. Primary role for IL-1beta and TNF-alpha in the triggering of preterm labor associated with inflammation or infection. PMID: 17132473
亞細胞定位：

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
蛋白家族：

Tumor necrosis factor family
數據庫鏈接：

KEGG: mcc:715467

STRING: 9544.ENSMMUP00000011594

UniGene: Mmu.3364

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.