Monkey Interferon γ,IFN-γ ELISA Kit

Monkey Interferon γ,IFN-γ ELISA Kit

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中文名稱：

猴γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒
貨號：

CSB-E10049Mo
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

猴γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒（CSB-E10049Mo）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNG含量。IFNG即干擾素-γ，在免疫調(diào)節(jié)等方面背景重要。它是由活化的T細(xì)胞和NK細(xì)胞產(chǎn)生的細(xì)胞因子。研究機(jī)制上，可激活巨噬細(xì)胞、增強(qiáng)MHC分子表達(dá)、調(diào)節(jié)Th1/Th2平衡等，在機(jī)體免疫防御、抗腫瘤等過程中發(fā)揮關(guān)鍵作用。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，支持科研人員開展非人靈長類動物模型的免疫機(jī)制研究、感染性疾病模型評估或藥物干預(yù)實驗中的細(xì)胞因子動態(tài)監(jiān)測本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
縮寫：

IFNG
Uniprot No.：

P63310
種屬：

Monkey
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	1.830	1.990	1.910	1.805
1000	1.496	1.398	1.447	1.342
500	1.133	1.138	1.136	1.031
250	0.932	0.902	0.917	0.812
125	0.544	0.535	0.540	0.435
62.5	0.212	0.226	0.219	0.114
31.25	0.117	0.111	0.114	0.009
0	0.106	0.104	0.105

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

平均分:

5.0分 - 1 個評價

樣品類型：細(xì)胞培養(yǎng)上清液

樣品信息：其他猴

稀釋比：其他 1：3，1：5

產(chǎn)品評價：我用CSB-E10049Mo檢測食蟹猴細(xì)胞培養(yǎng)上清液，標(biāo)曲OD值范圍為2.780-0.1055，樣品檢測OD值范圍為0.1274-0.0503，檢測結(jié)果低于下限，可能是稀釋倍數(shù)問題，標(biāo)曲正常。

By 榮老師

功能：

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
基因功能參考文獻(xiàn)：
1. IFNgamma expression in colonic epithelial cells was regulated by TGFB1. PMID: 25769244
2. Higher KIR2DL4 copy numbers is associated with an increased IFN-gamma production in NK cell subsets in SIV-infected Mamu-A*01-negative rhesus macaques. PMID: 23449795
3. Data show that viral set-point in simian immunodeficiency virus diseasewas is associated with expression of interferon gamma -stimulated genes. PMID: 17719201
4. Low Sociable animals showed alterations in lymph node expression of the immunoregulatory cytokine interferon-gamma. PMID: 18068331
亞細(xì)胞定位：

Secreted.
蛋白家族：

Type II (or gamma) interferon family
組織特異性：

Released primarily from activated T lymphocytes.
數(shù)據(jù)庫鏈接：

KEGG: mcc:574282

STRING: 9544.ENSMMUP00000025266

UniGene: Mmu.3373

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.