Human γ-Secretase ELISA kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人γ分泌酶酶聯免疫試劑盒（CSB-E13495h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的γ-Secretase含量。γ-Secretase是一種具有重要生物學意義的靶點。它參與多種生物過程，尤其在淀粉樣前體蛋白（APP）的裂解過程中發揮關鍵作用。相關研究聚焦于其作用機制，旨在探索通過調控γ-Secretase來干預與APP裂解異常相關的疾病，如阿爾茨海默病。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，該產品適用于神經科學領域的基礎研究，例如阿爾茨海默病動物模型的組織樣本分析、體外培養細胞中γ分泌酶活性調控實驗，或藥理學研究中抑制劑/激活劑對酶表達水平影響的動態監測。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

gamma-secretase
縮寫：

γ-Secretase
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human γ-Secretase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
Range %	86-97
1:2	Average %	97
Range %	91-103
1:4	Average %	95
Range %	89-100
1:8	Average %	94
Range %	89-98

回收率：

The recovery of human γ-Secretase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	98-101
EDTA plasma (n=4)	98	95-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.