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Human β-defensins 3 ELISA Kit

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產品描述：

人β-防御素3酶聯免疫試劑盒（CSB-E14187h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織培養上清液、組織勻漿樣本中的DEFB103A含量。DEFB103A，也稱為beta Defensin 3，是一種由中性粒細胞產生的抗菌肽，屬于β-防御素家族。該蛋白在免疫系統中發揮重要作用，具有抗菌、免疫調節和保護作用。其機制可能涉及抑制腫瘤生長和增殖，促進抗腫瘤免疫反應。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，可滿足科研實驗中定量分析hBD-3在疾病模型、免疫調控機制或藥物干預研究中的動態變化需求，為探索其在感染、自身免疫性疾病或腫瘤微環境中的作用提供可靠工具；適用于體外樣本的批量檢測，為研究人員評估hBD-3的生物學功能及相關通路研究提供高效解決方案本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。

別名：

BD 3 ELISA Kit; BD-3 ELISA Kit; BD3 ELISA Kit; beta 103 ELISA Kit; Beta defensin 103A ELISA Kit; Beta defensin 103A precursor ELISA Kit; Beta defensin 3 ELISA Kit; Beta-defensin 103 ELISA Kit; Beta-defensin 3 ELISA Kit; D103A_HUMAN ELISA Kit; DEF B3 ELISA Kit; DEFB 103 ELISA Kit; DEFB 103A ELISA Kit; DEFB 3 ELISA Kit; DEFB-3 ELISA Kit; DEFB103 ELISA Kit; DEFB103A ELISA Kit; DEFB103B ELISA Kit; DEFB3 ELISA Kit; Defensin ELISA Kit; Defensin beta 103A ELISA Kit; Defensin beta 3 ELISA Kit; Defensin like protein ELISA Kit; Defensin-like protein ELISA Kit; HBD 3 ELISA Kit; hBD-3 ELISA Kit; HBD3 ELISA Kit; HBP 3 ELISA Kit; HBP3 ELISA Kit; mBD3 ELISA Kit

縮寫：

DEFB103A

Uniprot No.：

P81534

種屬：

Homo sapiens (Human)

樣本類型：

serum, plasma, urine, cell culture supernates, tissue homogenates

檢測范圍：

125 pg/mL-8000 pg/mL

靈敏度：

31.25 pg/mL

反應時間：

1-5h

樣本體積：

50-100ul

檢測波長：

450 nm

研究領域：

Immunology

測定原理：

quantitative

測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-101
1:2	Average %	98
	Range %	92-104
1:4	Average %	92
	Range %	87-96
1:8	Average %	90
	Range %	86-94

回收率：

The recovery of human β-defensins 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-94
EDTA plasma (n=4)	102	98-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.339	2.508	2.424	2.270
4000	1.833	1.869	1.851	1.697
2000	1.185	1.198	1.192	1.038
1000	0.699	0.687	0.693	0.539
500	0.375	0.392	0.384	0.230
250	0.294	0.281	0.288	0.134
125	0.208	0.211	0.210	0.056
0	0.153	0.155	0.154

本試劑盒所含材料：

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human BD3 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled BD3 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

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本試劑盒不含材料：

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

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數據處理：

ELISA操作視頻及標曲繪制軟件下載

貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.