Human very low density lipoprotein(VLDL)ELISA Kit

產品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人極低密度脂蛋白(VLDL)酶聯免疫試劑盒（CSB-E17087h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VLDL含量。VLDL（極低密度脂蛋白）是一種運載內源性甘油三酯的脂蛋白。其水平異常與心血管疾病等密切相關。研究機制主要聚焦于VLDL在肝臟合成、代謝過程，以及其與受體相互作用，以明確其在脂質代謝紊亂和疾病發生發展中的具體作用。試劑盒檢測范圍為0.78 μg/mL-50 μg/mL，適用于科研領域中對脂代謝機制、心血管疾病動物模型的研究，也可用于藥物干預或基因編輯后體內VLDL水平變化的動態監測，為代謝相關疾病的機制探索及生物標志物篩選提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

VLDL
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 μg/mL-50 μg/mL
靈敏度：

0.195 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human VLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	97
	Range %	91-103
1:400	Average %	95
	Range %	91-99
1:800	Average %	94
	Range %	88-98
1:1600	Average %	105
	Range %	101-109

回收率：

The recovery of human VLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	103	98-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.