Human upper zone of growth plate and cartilage matrix associated (UCMA) ELISA kit

Human upper zone of growth plate and cartilage matrix associated (UCMA) ELISA kit

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中文名稱：

人生長板上部區域與軟骨基質相關蛋白(UCMA)酶聯免疫試劑盒
貨號：

CSB-EL025550HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人生長板上部區域與軟骨基質相關蛋白(UCMA)酶聯免疫試劑盒（CSB-EL025550HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的UCMA含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于基礎科研領域，如探究骨骼發育異常、骨關節炎、軟骨代謝紊亂等疾病的分子機制，或用于體外模型中軟骨相關蛋白表達水平的動態監測，為組織工程、藥物篩選等研究提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

UCMA ELISA Kit; C10orf49 ELISA Kit; Unique cartilage matrix-associated protein [Cleaved into: Unique cartilage matrix-associated protein C-terminal fragment ELISA Kit; Ucma-C ELISA Kit; Gla-rich protein ELISA Kit; GRP)] ELISA Kit
縮寫：

UCMA
Uniprot No.：

Q8WVF2
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Developmental Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human UCMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	95
	Range %	91-99
1:4	Average %	96
	Range %	93-99
1:8	Average %	87
	Range %	82-93

回收率：

The recovery of human UCMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-89
EDTA plasma (n=4)	83	80-86

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.578	2.596	2.587	2.394
5	2.168	2.115	2.142	1.949
2.5	1.536	1.512	1.524	1.331
1.25	0.969	0.958	0.964	0.771
0.625	0.598	0.572	0.585	0.392
0.312	0.455	0.456	0.456	0.263
0.156	0.371	0.398	0.385	0.192
0	0.195	0.190	0.193

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Association of UCMA levels in serum and synovial fluid with severity of knee osteoarthritis Okuyan HM, et al,International Journal of Rheumatic Diseases,2019

功能：

May be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells in peripheral zones of fetal cartilage and at the cartilage-bone interface.
基因功能參考文獻：
1. GRP might act as a novel molecular mediator linking inflammation and calcification events PMID: 28542410
2. The study demonstrates the involvement of GRP in the two major molecular processes affecting osteoarthritis: mineralization and inflammation of articular tissue. PMID: 26337479
3. Data suggest that novel splice variants of GRP are expressed in cartilage and other tissues (i.e. GRP-F5, GRP-F6); GRP-F1 is predominant splice variant expressed in adult tissues particularly in cartilage of patients with osteoarthritis. PMID: 24867294
4. full exon resequencing of DNA samples from 17 chronic kidney disease patients (stage 5) and 121 healthy controls in a Swsedish population; the 138Thr > Ser polymorphism seems to be the only non-synonymous SNP found in the UCMA gene in a Swedish population PMID: 24409676
5. Gla-rich protein (GRP) is a new vitamin K-dependent protein that can bind calcium through Gla residues. PMID: 18836183
6. The present data strongly suggest an important function of Ucma in the early phase of chondrocyte differentiation. PMID: 17707622
7. Ucma may be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells PMID: 18156182
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亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

UCMA family
組織特異性：

Predominantly expressed in resting chondrocytes.
數據庫鏈接：

HGNC: 25205

KEGG: hsa:221044

STRING: 9606.ENSP00000367952

UniGene: Hs.112998

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.