Human syndecan 4 (SDC4) ELISA kit

產品詳情

文獻引用 (1) 評價 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人多配體聚糖4(SDC4)酶聯免疫試劑盒（CSB-EL020891HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、細胞裂解物、組織勻漿樣本中的SDC4含量。SDC4（Syndecan-4）是一種位于人類染色體20q13.12上的蛋白質編碼基因，編碼的蛋白在肝、肺等組織中廣泛表達。SDC4與細胞骨架蛋白結合和纖連蛋白結合相關，參與細胞外基質降解和信號傳導。其研究機制涉及細胞與細胞外基質相互作用，在血管損傷后新生內膜形成、腫瘤侵襲等過程中發揮重要作用。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于探究SDC4在腫瘤生物學行為、炎癥微環境調控或細胞基質相互作用中的分子機制，也可用于疾病模型構建過程中生物標志物的動態監測，為細胞信號傳導、組織修復等基礎研究領域提供數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

SDC4
Uniprot No.：

P31431
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell lysates, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SDC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	92
	Range %	90-96
1:4	Average %	93
	Range %	85-100
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of human SDC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	94	88-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 1 個評價

樣品類型：血漿（抗凝劑） EDTA

樣品信息：人

稀釋比：沒有稀釋

產品評價： ELISA結果正常組和患病組有明顯差異

By 劉老師

功能：

Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
亞細胞定位：

[Isoform 1]: Membrane; Single-pass type I membrane protein. Secreted.; [Isoform 2]: Secreted.
蛋白家族：

Syndecan proteoglycan family
組織特異性：

Expressed in epithelial and fibroblastic cells.
數據庫鏈接：

HGNC: 10661

OMIM: 600017

KEGG: hsa:6385

STRING: 9606.ENSP00000361818

UniGene: Hs.632267