Human serum amyloid A2 (SAA2) ELISA kit
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中文名稱:人血清淀粉樣蛋白A2(SAA2)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-EL020657HU
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人血清淀粉樣蛋白A2(SAA2)酶聯(lián)免疫試劑盒(CSB-EL020657HU)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的SAA2含量。SAA2即血清淀粉樣蛋白A2,屬于急性時(shí)相蛋白。在炎癥、感染等刺激下其表達(dá)水平會(huì)急劇上升。研究機(jī)制方面,它參與免疫調(diào)節(jié),可趨化免疫細(xì)胞。還與代謝紊亂、心血管疾病等發(fā)病機(jī)制有一定聯(lián)系,是潛在的疾病診斷與治療靶點(diǎn)。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL,為探索炎癥相關(guān)疾病機(jī)制(如動(dòng)脈粥樣硬化、類風(fēng)濕性關(guān)節(jié)炎等)提供可靠工具,亦可用于藥物干預(yù)或基因編輯模型中SAA2表達(dá)變化的科研評(píng)估,滿足體外實(shí)驗(yàn)、動(dòng)物模型及細(xì)胞研究等多場(chǎng)景需求。適用于血清、血漿等體液樣本中SAA2的動(dòng)態(tài)監(jiān)測(cè),或組織勻漿中蛋白表達(dá)的定量分析。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:N/A
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縮寫:SAA2
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:0.312 ng/mL-20 ng/mL
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靈敏度:0.078 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Cardiovascular
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human SAA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:50
Average %
93
Range %
88-99
1:100
Average %
89
Range %
82-96
1:200
Average %
99
Range %
94-105
1:400
Average %
92
Range %
86-98
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回收率:
The recovery of human SAA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
98
91-104
EDTA plasma (n=4)
91
84-97
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
20
2.175
2.234
2.205
2.066
10
1.784
1.667
1.726
1.587
5
1.303
1.308
1.306
1.167
2.5
0.829
0.869
0.849
0.710
1.25
0.577
0.601
0.589
0.450
0.625
0.432
0.453
0.443
0.304
0.312
0.267
0.280
0.274
0.135
0
0.143
0.135
0.139
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Label-Free Quantitative Proteomics Identifies Novel Biomarkers for Distinguishing Tuberculosis Pleural Effusion from Malignant Pleural Effusion Pan L, et al,Proteomics??linical Applications,2019
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