Human oxyntomodulin ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人胃泌酸調(diào)節(jié)素酶聯(lián)免疫試劑盒（CSB-E12948h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、尿液、組織勻漿樣本中的OXM含量。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，適用于體外研究場(chǎng)景下OXM的表達(dá)水平分析，可為能量代謝調(diào)控機(jī)制研究、胃腸道功能探索及代謝性疾病分子機(jī)制研究提供可靠數(shù)據(jù)支持，滿足基礎(chǔ)科研與藥物研發(fā)中對(duì)OXM定量檢測(cè)的需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

OXM
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, urine, tissue homogenates
檢測(cè)范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-94
1:2	Average %	84
	Range %	80-89
1:4	Average %	95
	Range %	89-99
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of human Oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	98	92-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.