Human osteocrin,Ostn ELISA kit

Human osteocrin,Ostn ELISA kit

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中文名稱：

人骨織素(Ostn)酶聯(lián)免疫試劑盒
貨號：

CSB-E12021h
規(guī)格：

96T/48T
價格：

￥3800/￥2500
促銷：
其他：

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人骨織素(Ostn)酶聯(lián)免疫試劑盒（CSB-E12021h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的OSTN含量。人骨織素（Ostn）是一種由骨骼細胞分泌的糖蛋白，參與骨代謝調(diào)控和能量穩(wěn)態(tài)維持。其研究機制涉及調(diào)節(jié)細胞粘附、遷移、增殖和分化等生物學功能，通過整合素受體激活細胞內(nèi)信號通路，影響細胞生長和行為。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于基礎科研場景，如探索骨骼疾病機制、評估骨代謝相關生物標志物或研究能量代謝調(diào)控網(wǎng)絡。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

OSTN ELISA Kit; Osteocrin ELISA Kit; Musclin) [Cleaved into: Processed Osteocrin] ELISA Kit
縮寫：

OSTN
Uniprot No.：

P61366
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Stem Cells
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Ostn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	99
	Range %	94-103
1:4	Average %	96
	Range %	92-99
1:8	Average %	95
	Range %	88-98

回收率：

The recovery of human Ostn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	97	91-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.552	2.699	2.626	2.510
10	2.254	2.373	2.314	2.198
5	1.804	1.928	1.866	1.750
2.5	0.889	0.926	0.908	0.792
1.25	0.517	0.498	0.508	0.392
0.625	0.320	0.304	0.312	0.196
0.312	0.216	0.202	0.209	0.093
0	0.118	0.114	0.116

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

The muscle-enriched myokine Musclin impairs beige fat thermogenesis and systemic energy homeostasis via Tfr1/PKA signaling in male mice L Jin,Nature communications,2023
Low Circulating Musclin is Associated With Adverse Prognosis in Patients Undergoing Transcatheter Aortic Valve Implantation at Low‐Intermediate Risk B Kattih,Journal of the American Heart Association,2022

功能：

Hormone that acts as a regulator of dendritic growth in the developing cerebral cortex in response to sensory experience. Induced in the brain following membrane depolarization and inhibits dendritic branching in neurons of the developing cortex. Probably acts by binding to natriuretic peptide receptor NPR3/NPR-C, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production.
基因功能參考文獻：
1. The circulating concentration of musclin was significantly increased in type 2 diabetes mellitus patients. Results suggest that musclin has a strong relationship with insulin resistance in type 2 diabetes mellitus. PMID: 28185530
2. findings suggest that, in response to sensory input, OSTN regulates features of neuronal structure and function that are unique to primates PMID: 27830782
3. osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator PMID: 14523025
4. Foxo1 downregulates musclin mRNA expression both in vitro and in vivo, which should explain insulin-mediated upregulation of this gene in muscle cells. PMID: 17950246
5. Ostn is a naturally occurring ligand of the NPR-C clearance receptor and may act to locally modulate the actions of the natriuretic system in bone by blocking the clearance action of NPR-C, thus locally elevating levels of C-type natriuretic peptide. PMID: 17951249
6. Observational study of gene-disease association. (HuGE Navigator) PMID: 19453261
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亞細胞定位：

Secreted.
蛋白家族：

Osteocrin family
組織特異性：

Enriched in neocortical regions of the developing cerebral cortex. Not expressed in other compartments of the neocortical wall or in brain regions such as the hippocampus, striatum, mediodorsal nucleus of the thalamus and cerebellum. Also expressed in bon
數(shù)據(jù)庫鏈接：

HGNC: 29961

OMIM: 610280

KEGG: hsa:344901

STRING: 9606.ENSP00000342356

UniGene: Hs.526794

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.