Human orosomucoid 2,ORM2 ELISA Kit

Human orosomucoid 2,ORM2 ELISA Kit

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中文名稱：

人類粘蛋白2(ORM2)酶聯免疫試劑盒
貨號：

CSB-E11821h
規格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人類粘蛋白2(ORM2)酶聯免疫試劑盒（CSB-E11821h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ORM2含量。試劑盒檢測范圍為78 ng/mL-5000 ng/mL，靈敏度為19.5 ng/mL。適用于科研領域中對ORM2蛋白表達水平的定量分析，例如探索炎癥相關疾病機制、評估代謝綜合征生物標志物或研究肝臟功能異常模型中的蛋白動態變化本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

ORM2 ELISA Kit; AGP2Alpha-1-acid glycoprotein 2 ELISA Kit; AGP 2 ELISA Kit; Orosomucoid-2 ELISA Kit; OMD 2 ELISA Kit
縮寫：

ORM2
Uniprot No.：

P19652
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

78 ng/mL-5000 ng/mL
靈敏度：

19.5 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ORM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	90-102
1:200	Average %	89
	Range %	83-94
1:400	Average %	101
	Range %	93-105
1:800	Average %	105
	Range %	97-110

回收率：

The recovery of human ORM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-95
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
5000	0.131	0.135	0.133
2500	0.389	0.401	0.395
1250	0.750	0.732	0.741
625	1.042	1.020	1.031
312	1.441	1.454	1.448
156	1.650	1.666	1.658
78	1.806	1.767	1.787
0	2.001	2.078	2.040

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

最新研究進展：

ORM2，全稱為酸性糖蛋白，是一種血漿蛋白。最近的研究表明，ORM2在多種疾病中發揮著重要作用，包括炎癥、心血管疾病和肝病等。ORM2參與了細胞凋亡、免疫反應和糖代謝等生物學過程。
功能：

Functions as transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
基因功能參考文獻：
1. the glycosylation change of alpha-1-acid glycoprotein (AGP) in hepatocellular carcinoma (HCC), cirrhosis and controls PMID: 28621608
2. Neural cell interactions are important for brain physiology and pathology. Particularly, the interaction between non-neuronal cells plays a central role in regulating brain inflammation, which is closely linked to many brain disorders. Here, we newly identified orosomucoid-2 (ORM2) as an endogenous protein that mediates such non-neuronal glial cell interactions PMID: 28193696
3. Low ORM2 expression is associated with metastasis in hepatocellular carcinoma. PMID: 25965830
4. Data suggest that human serum albumin (HSA) might serve as a carrier in delivering chitooligomers to target tissues than alpha-1-glycoprotein (AGP) which has pharmacological importance. PMID: 24359035
5. Alteration in expression of ORM2 suggests that ORM2 could be used as a potential biomarker in the diagnosis of colorectal cancer. PMID: 22363757
6. the sera of patients with acute inflammation demonstrated increased numbers of bi-antennary and alpha1,3-fucosylated N-glycan structures at each glycosylation site PMID: 15863355
7. The binding of coumarin enantiomers to ORM2 is studied. PMID: 16290938
8. Results describe the thermal unfolding and reversibility of temperature-induced changes in alpha(1)-acid glycoprotein. PMID: 16331959
9. The results indicate that, in accordance with prior expectations, the ORM2 variant is responsible for the acute-phase property of alpha-1 acid glycoprotein. PMID: 19018521
10. structure of the alpha1-acid glycoprotein (AGP), or orosomucoid (ORM), gene was investigated in a Ghanaian mother and her child, who shared an unusual variant, ORM1 S2(C), found by isoelectric focusing PMID: 11587070
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亞細胞定位：

Secreted.
蛋白家族：

Calycin superfamily, Lipocalin family
組織特異性：

Expressed by the liver and secreted in plasma.
數據庫鏈接：

HGNC: 8499

OMIM: 138610

KEGG: hsa:5005

STRING: 9606.ENSP00000394936

UniGene: Hs.719954

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.