Human melanin-concentrating hormone (PMCH) ELISA kit

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中文名稱：

人黑色素濃縮激素(PMCH)酶聯免疫試劑盒
貨號：

CSB-EL018230HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人黑色素濃縮激素(PMCH)酶聯免疫試劑盒（CSB-EL018230HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PMCH含量。PMCH即前黑素聚集激素，它是一種神經肽。在其與能量代謝、睡眠覺醒等生理過程相關。研究機制上，主要是通過與特定受體結合，激活細胞內信號通路來發揮作用，目前在肥胖、睡眠障礙等病癥的治療研究中受關注。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于探索PMCH在能量代謝調控、神經內分泌系統中的作用機制，或用于評估疾病模型、藥物干預實驗中的PMCH動態變化。該產品嚴格適配科研場景，為代謝性疾病、神經科學等領域的基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

PMCH ELISA Kit; MCHPro-MCH [Cleaved into: Neuropeptide-glycine-glutamic acid ELISA Kit; NGE ELISA Kit; Neuropeptide G-E); Neuropeptide-glutamic acid-isoleucine ELISA Kit; NEI ELISA Kit; Neuropeptide E-I); Melanin-concentrating hormone ELISA Kit; MCH)] ELISA Kit
縮寫：

PMCH
Uniprot No.：

P20382
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PMCH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	87
	Range %	83-91
1:4	Average %	92
	Range %	88-96
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of human PMCH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-104
EDTA plasma (n=4)	84	80-88

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.682	2.533	2.608	2.400
500	2.040	1.946	1.993	1.785
250	1.419	1.357	1.388	1.180
125	0.927	0.975	0.951	0.743
62.5	0.654	0.689	0.672	0.464
31.2	0.408	0.392	0.400	0.192
15.6	0.335	0.324	0.330	0.122
0	0.204	0.211	0.208

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Effects of mild cognitive impairment and sleep disorders on the minimum alveolar concentration value of sevoflurane L Wang, S Yang, Z Niu, Y Guo,BMC anesthesiology,2024

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功能：

MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.
基因功能參考文獻：
1. MCH is an anabolic neuropeptide with multiple and diverse physiological functions including a key role in energy homoeostasis; evidence suggests that, in the reproductive context, MCH is a modulator rather than a primary regulator. PMID: 23884861
2. Cerebrospinal fluid (CSF) levels were measured in 33 patients with Alzheimer's disease (AD) and 33 healthy subjects, to investigate the role of MCH and HCRT-1 in AD. PMID: 23667582
3. the changes in the levels of two hypothalamic neuropeptides, hypocretin-1 and melanin-concentrating hormone, measured in the human amygdala, are reported. PMID: 23462990
4. Melanin-concentrating hormone (MCH) neurons of human hypothalamus, as well as the MCH neurons of mouse hypothalamus, contain IL-6Ralpha. PMID: 22295972
5. PMCH mRNA expression level was increased 1.85 times (P < 0.01) in vitiligo uninvolved skin and 5.36 times (P < 0.01) in involved skin compared to healthy control skin. PMID: 20888736
6. Melanin-concentrating hormone is expressed and functional in human skin PMID: 12176038
7. RT-PCR analysis revealed a low expression of prepro-MCH and MCH receptor 1 (MCHR1) but not of MCHR2 transcript in tissular and peripheral blood immune cells PMID: 12220661
8. Review. Melanin-concentrating hormone (MCH) is a cyclic neuropeptide, which centrally regulates food intake and stress. MCH-R1 antagonists can probe additional functions of the peptide, both in the brain and in the peripheral nervous system. PMID: 12885269
9. Serum levels could serve as useful peripheral markers of changes in energy homeostasis. PMID: 15546902
10. hMCH increased water intake independent of food intake. It significantly increased both sucrose solution and food intake. PMID: 15870910
11. Single nucleotide polymorphisms in pro-melanin-concentrating hormone may be associated with obesity and a greater body mass index in olanzapine-treated schizophrenic patients PMID: 17541984
12. expression of PMCH by activated Th2 cells in vivo may directly link allergic inflammation to energy homeostasis and may contribute to the association between asthma and obesity PMID: 17640905
13. The total number of hypocretin-1 neurons was significantly reduced by 30% in HD brains (P = 0.015), while the total number of MCH neurons was not significantly altered (P = 0.100). PMID: 18498421
14. MCH has a role in inflammatory processes in the intestine PMID: 18650383
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亞細胞定位：

Secreted.
蛋白家族：

Melanin-concentrating hormone family
組織特異性：

Predominantly expressed in lateral hypothalamus, also detected in pallidum, neocortex and cerebellum. Also found in thymus, brown adipose tissue, duodenum and testis (spermatogonia, early spermatocytes and Sertoli cells). No expression in peripheral blood
數據庫鏈接：

HGNC: 9109

OMIM: 176795

KEGG: hsa:5367

STRING: 9606.ENSP00000332225

UniGene: Hs.707990

Human melanin-concentrating hormone (PMCH) ELISA kit

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