Human matrix extracellular phosphoglycoprotein,MEPE ELISA Kit

Human matrix extracellular phosphoglycoprotein,MEPE ELISA Kit

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中文名稱：

人細胞外基質磷酸糖蛋白(MEPE)酶聯免疫試劑盒
貨號：

CSB-E09173h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人細胞外基質磷酸糖蛋白(MEPE)酶聯免疫試劑盒（CSB-E09173h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MEPE含量。MEPE（基質細胞外磷酸糖蛋白）是一種由MEPE基因編碼的蛋白質，屬于SIBLING蛋白家族，參與骨骼和牙質細胞外基質的構成與骨礦化調節。其研究機制涉及成骨細胞表達、受1,25-(OH)2D3調節，并在牙齒和骨骼發育中發揮重要作用。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，適用于體外研究MEPE在骨代謝疾病模型中的表達調控、礦化相關信號通路機制探索，或評估不同處理條件下（如藥物干預、基因修飾）生物樣本中MEPE的濃度變化，為骨生物學、礦物質代謝等領域的科研工作提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Matrix extracellular phosphoglycoprotein ELISA Kit; MEPE ELISA Kit; MEPE_HUMAN ELISA Kit; OF45 ELISA Kit; Osteoblast/osteocyte factor 45 ELISA Kit
縮寫：

MEPE
Uniprot No.：

Q9NQ76
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 ng/mL-1000 ng/mL
靈敏度：

3.9 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MEPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	89
	Range %	84-93
1:10	Average %	98
	Range %	95-102
1:20	Average %	84
	Range %	80-88
1:40	Average %	90
	Range %	85-94

回收率：

The recovery of human MEPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	105	99-110

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.603	2.769	2.686	2.516
500	1.967	2.075	2.021	1.851
250	1.290	1.194	1.242	1.072
125	0.631	0.679	0.655	0.485
62.5	0.476	0.506	0.491	0.321
31.2	0.341	0.357	0.349	0.179
15.6	0.254	0.246	0.250	0.080
0	0.170	0.169	0.170

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Promotes renal phosphate excretion and inhibits intestinal phosphate absorption. Promotes bone mineralization by osteoblasts and cartilage mineralization by chondrocytes. Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage. Promotes dental pulp stem cell proliferation and differentiation.
基因功能參考文獻：
1. Results indicate an association of MEPE gene inactivation with decreased survival after DNA damage. PMID: 23570370
2. Mice deficient in an ortholog of MEPE show increased bone mass and are resistant to aging-related bone loss. PMID: 12421822
3. results indicated that MEPE appeared to play an important positive role in proliferation and osteogenesis differentiation of DPCs through interaction with downstream signals. PMID: 22341070
4. MEPE or the MEPE-derived acidic serine aspartate-rich MEPE-associated motif peptide may contribute to decreased bone mineralization in Oncogenic osteomalacia patients. PMID: 21739089
5. Among migraineurs with aura rs7698623 in MEPE (OR = 6.37; 95% CI 3.15-12.90; p = 2.7x10(-7)) and rs4975709 in IRX4 (OR = 5.06; 95% CI 2.66-9.62; p = 7.7x10(-7)) appeared to be associated with ischemic stroke. PMID: 21779381
6. Sclerostin acts through regulation of the PHEX/MEPE axis at the preosteocyte stage and serves as a master regulator of physiologic bone mineralization. PMID: 21312267
7. MEPE may play a regulatory role in periodontal ligament cell osteogenic differentiation. PMID: 19031789
8. These results suggest that abnormal MEPE cleavage occurs when PHEX activity is deficient in humans. PMID: 20581062
9. This study demonstrates the importance of posttranslational modification for the site-specific activity of MEPE and its ASARM peptide. PMID: 19998030
10. The patterns of expression of FGF-23, MEPE, and DMP1 differ markedly in trabecular bone, suggesting that individual osteocytes may have specialized functions. PMID: 19679205
11. MEPE/OF45, a new dentin/bone matrix protein and candidate gene for dentin diseases mapping to chromosome 4q21. Expressed during odontogenesis. PMID: 12489176
12. There is evidence for a hormone/enzyme/extracellular matrix protein cascade involving fibroblastic growth factor 23 (FGF23), a phosphate-regulating gene with homologies to (PHEX) and (MEPE)--REVIEW PMID: 12791601
13. MEPE is predominantly expressed by osteocytes in human bone, with significant expression by osteocytes within mineralized bone PMID: 15108058
14. The results are consistent with MEPE being involved in phosphate and bone metabolism in a normal population. PMID: 15292364
15. MEPE expression is higher when cells proliferate, whereas it is downregulated as cells differentiated PMID: 18547474
16. MEPE is a candidate phosphatonin involved in phosphate homeostasis, acting in both the kidney and the gastrointestinal tract PMID: 19005008
17. MEPE is expressed in salivary gland ducts. PMID: 15329369
18. MEPE is expressed on the luminal surface of kidney proximal tubules. PMID: 15954904
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亞細胞定位：

Secreted, extracellular space, extracellular matrix.
組織特異性：

Expressed by osteoblasts. Expressed by stem cells in dental pulp. Expressed by mesenchymal cells in dental papilla and dental pulp. Expressed in teeth, specifically in decidious dentin. Expressed in ondotoblasts. Expressed in salivary glands. Secreted fro
數據庫鏈接：

HGNC: 13361

OMIM: 605912

KEGG: hsa:56955

STRING: 9606.ENSP00000354341

UniGene: Hs.676357

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.