Human histatin 5 ELISA Kit

產品詳情

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文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人富組蛋白(histatin-5)酶聯免疫試劑盒（CSB-E09935h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、唾液樣本中的histatin-5含量。Histatin-5是一種由唾液腺分泌的低分子量蛋白質，具有抗菌和抗真菌活性。其研究機制主要涉及抑制細菌和真菌的細胞膜完整性，干擾其代謝和復制過程，以及調節宿主的免疫反應。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，適用于研究口腔微生物感染、炎癥反應調控或免疫調節機制等科研領域；可用于評估口腔疾病模型、抗菌肽功能研究或唾液成分分析，為探索口腔黏膜免疫及宿主 - 微生物互作提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

histatin-5
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, saliva
檢測范圍：

12.5 ng/mL-800 ng/mL
靈敏度：

3.12 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Histatin 5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	94
	Range %	90-98
1:4	Average %	100
	Range %	95-104
1:8	Average %	102
	Range %	96-108

回收率：

The recovery of human Histatin 5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	95	91-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.