- A 96-well Assay plate --The 96-well plate has been pre-coated with HS.
- Standard (2 x 250 μl)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- Anti-human HS Antibody (100 x concentrate) (1 x 60 μl)
- Antibody Diluent (1 x 10 ml) --Dilute the anti-human LRG1
- HRP-conjugated goat-anti-mouse HS antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.
- HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated HS antibody.
- Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
- Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
- TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
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