Human growth differentiation factor-9,GDF-9 ELISA Kit
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中文名稱:人生長分化因子-9(GDF-9)酶聯免疫試劑盒
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貨號:CSB-E12925h
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:人生長分化因子-9(GDF-9)酶聯免疫試劑盒(CSB-E12925h)為雙抗夾心法ELISA試劑盒,定量檢測樣本中的GDF9含量。GDF9屬于生長分化因子家族,在卵泡發育中起關鍵作用。研究發現其能促進顆粒細胞增殖、調節基因表達,參與卵泡從原始到竇前階段的轉變。深入了解GDF9作用機制,有望為女性生殖疾病診治及生殖調控提供新靶點與思路。支持科研人員開展卵巢功能評估、生殖疾病分子機制探索、生育力相關動物模型研究等實驗;可為生殖生物學、內分泌學及發育生物學等方向提供可靠的數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:GDF9Growth/differentiation factor 9 ELISA Kit; GDF-9 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:
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檢測范圍:
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靈敏度:
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Cardiovascular
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
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線性度:
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回收率:
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標準曲線:
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數據處理:
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貨期:3-5 working days
引用文獻
- GDF9His209GlnfsTer6/S428T and GDF9Q321X/S428T bi-allelic variants caused female subfertility with defective follicle enlargement Y Duan,Cell communication and signaling,2024
- Hormonal Profile of Follicular Fluid between Mild Stimulation and Controlled OvarianHyperstimulation IVF Cycles in POR Patients LI Ting-ting.et al,J SUN Yat-sen Univ,2018
相關產品
相關問答
■ 常見問題解答
靶點詳情
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功能:Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.
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基因功能參考文獻:
- The present mini-review provides an overview of recent findings relating GDF-9 and BMP-15 as fundamental factors implicated in the regulation of ovarian function and discusses their potential role as markers of oocyte quality in women. PMID: 30039232
- Data suggest that GDF9 and BMP15 are oocyte-secreted factors with leading roles in control of ovarian function in female reproduction, modulating both fate of somatic granulosa cells and quality/developmental competence of the egg. (GDF9 = growth and differentiation factor-9; BMP15 = bone morphogenetic protein-15) [REVIEW] PMID: 29544636
- GDF9 single nucleotide polymorphism is not associated with Endometriosis-associated infertility. PMID: 28831646
- GDF9 single nucleotide polymorphism is associated with fecundity. PMID: 28762037
- Asiaticoside hindered the invasive growth of KFs by inhibiting the GDF-9/MAPK/Smad pathway. PMID: 28346732
- Human GDF9 and BMP15 act synergistically to stimulate granulosa cell proliferation, a response that also involves species-specific non-SMAD signalling pathways. PMID: 25155366
- these results indicated that the GDF9 C447T SNP is a possible candidate genetic risk factor for female infertility in the Polish population. PMID: 27035733
- GDF-9 promotes the proliferation and migration of keloid fibroblasts via a mechanism involving the Smad2/3 pathway. PMID: 27855376
- GDF9 c.169G>T (D57Y), c.546G>A (rs1049127), and BMP15 rs79377927 (788_789insTCT) were associated with premature ovarian failure in the Chinese Hui population. PMID: 25954833
- results support a model of activation for human GDF9 dependent on cumulin formation through heterodimerization with BMP15. Oocyte-secreted cumulin is likely to be a central regulator of fertility in mono-ovular mammals. PMID: 26254468
- GDF-9 and GDF-15 levels may influence bone parameters in polycystic ovary syndrome PMID: 24430093
- GDF9 and BMP15 expression is reduced in primordial, primary, and secondary follicles in ovarian tissues of PCOS patients. PMID: 25172094
- The arginine residue in the pre-helix loop of GDF9 homodimer may prevent the inhibition from its pro-domain or directly alter receptor binding, but this residue in GDF9 does not significantly affect the heterodimer activity. PMID: 25253739
- Eleven unique copy number changes are identified in a total of 11 patients, including a tandem duplication of 475 bp, containing part of the GDF9 gene promoter region. PMID: 24939957
- Data suggest up-regulation of mRNA for GDF9 and BMP15 (bone morphogenetic protein 15) in cumulus granulosa cells serves as biomarker for oocyte maturation, fertilization, embryo quality, and pregnancy in couples undergoing ICSI for male infertility. PMID: 25139161
- These results suggest that COCs cultured with recombinant GDF9 in oocyte maturation medium improve oocyte developmental competence and subsequent developmental competence of cloned embryo in cattle. PMID: 24840335
- Reduced expression of growth and differentiation factor-9 is associated with aggressive behaviour of clear-cell renal cell carcinoma. PMID: 25368253
- Oocyte-derived GDF9 does not alter gap junction intercellular communication activity between human granulosa cells. PMID: 24413384
- Alterations to hGDF9 synthesis and activity can contribute to the most common ovarian pathologies. PMID: 24438375
- genetic association studies in population of Han women in China: Data suggest that a mutation in precursor region of GDF9 (R146C) is associated with premature ovarian failure (POF); R146C mutation found in 3 women with POF but absent in controls. PMID: 23851219
- FOXL2 expression is required for GDF-9 stimulation of follistatin transcription. PMID: 23523567
- BMP15 and GDF9 transcript levels increase in mature oocytes from women with polycystic ovary syndrome after ovarian stimulation, and might inhibit the progesterone secretion by follicular cells PMID: 22825968
- findings that GDF9:BMP15 heterodimers are the most bioactive ligands in mice and humans compared with homodimers explain many puzzling genetic and physiological data and have important implications for improving female fertility in mammals PMID: 23382188
- CDC6 and GDF-9 might be closely related to the carcinogenesis, clinical biological behaviors, and prognosis of gallbladder adenocarcinoma. PMID: 23011157
- It was shown that human kidney tumours have a reduced or loss of expression of GDF9. In vitro, GDF9 overexpression suppresses the invasiveness, growth and migration of kidney cancer cells. PMID: 23060562
- GDF-9 levels are inversely correlated with the growth, adhesion and migration of bladder cancer cells in vitro. PMID: 22159313
- GDF9 may contribute to the variation observed in follicular development, ovulation rate, and fecundity between mammals PMID: 22234469
- GDF9 decreases basal and activin A-induced FST and FSTL3 expression, and this explains, in part, its enhancing effects on activin A-induced inhibin beta(B)-subunit mRNA expression and inhibin B production in hGL cells PMID: 21829661
- In the samples from girls/women, the number of developing follicles was greater with GDF9 or BMP15 alone than with no BMP15 or GDF9. PMID: 21632818
- The expression of GDF9 and BMP15 in oocytes from patients with polycystic ovary syndrome cannot reach the normal level even after ovarian stimulation. PMID: 21669410
- Higher mature GDF9 levels in the follicular fluid were significantly correlated with oocyte nuclear maturation and embryo quality. PMID: 21496799
- GDF-9 signaling via ALK-5, can promote cell invasiveness PMID: 21116689
- Integral role of GDF-9 and BMP-15 in ovarian function. PMID: 21226076
- GDF-9 can promote the motile and adhesive capacity of PC-3 prostate cancer cells by up-regulating expression of FAK and paxillin in a Smad dependent manner, suggesting a pro-tumourigenic role for GDF-9 in prostate cancer. PMID: 21042764
- Impaired production of BMP15 and GDF9 mature proteins derived from proproteins with mutation in the proregion. PMID: 20547206
- Mutational analysis of the coding region of GDF9 among 216 Chinese polycystic ovary syndrome patients. Five novel missense mutations were discovered namely c.15C>G, c.118T>G, c.133A>G, c.1025A>T and c.1275C>A. PMID: 20705511
- The detection of GDF9 and TGFbetaR1 at both at the protein and mRNA levels suggests that GDF9 may have functions in human preantral follicles. PMID: 20427239
- GDF-9 c.G546A, but not c.G169A or c.C447T, is correlated with the poor ovarian stimulation and in vitro fertilization outcomes in women with diminished ovarian reserve. PMID: 20451184
- GDF9 attenuates the suppressive effects of activin A on StAR expression and progesterone production by increasing the expression of inhibin B, which acts as an activin A competitor. PMID: 20660033
- Cell growth was significantly increased in the GDF9 over-expressing cells compared to the two controls; apoptosis was decreased in the presence of GDF9. PMID: 20458753
- GDF-9 concentration in women with severe endometriosis was lower than in those without PMID: 19931079
- data demonstrate that polymorphisms in major folliculogenesis genes, GDF9, BMP15, AMH, and AMHR2, are not associated with polycystic ovary syndrome susceptibility. PMID: 20236105
- Reduced GDF-9 expression in cumulus granulosa cells of patients with polycystic ovary syndrome (PCOS) appears to be associated with decreased long-term developmental potential of the oocytes of patients with PCOS. PMID: 19376510
- Data show that Golgi apparatus casein kinase (G-CK) catalyzes the phosphorylation of rhBMP-15 and rhGDF-9. PMID: 20067794
- 2 synonymous mutations (c.447C>T, c.546G>A) & a novel missense variant (c.169G>T) were found in both premature ovarian failure patients & control subjects in the coding region of GDF9, indicating them as a novel polymorphism PMID: 19438907
- Bone morphogenetic protein receptor type II is a receptor for growth differentiation factor-9 PMID: 12135884
- interaction with bone morphogenetic protein-15 PMID: 12446716
- BMP-15 and GDF-9 have roles in fertility; critical sequences are determined by mutagenesis PMID: 14970198
- This case-control study revealed eight mutations in the GDF9 gene in women with premature ovarian failure PMID: 16278619
- although mutations in BMP15 and GDF9 are not a major cause of ovarian insufficiency, they may be involved in premature ovarian failure PMID: 16645022
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相關疾病:Altered GDF9 function may be involved in ovarian disorders. Rare variants in GDF9 have been found in patients with premature ovarian failure and mothers of dizygotic twins.
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亞細胞定位:Secreted.
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蛋白家族:TGF-beta family
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組織特異性:Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level).
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