Human flagellin ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人鞭毛蛋白(flagellin)酶聯(lián)免疫試劑盒（CSB-E09671h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的flagellin含量。鞭毛蛋白（flagellin）是細(xì)菌鞭毛的主要組成部分。它能被宿主模式識(shí)別受體識(shí)別，激活先天性免疫反應(yīng)。相關(guān)研究聚焦于其與免疫細(xì)胞的相互作用機(jī)制，探索將其作為免疫佐劑、疫苗抗原等，以增強(qiáng)免疫效果、防治感染等疾病。試劑盒檢測(cè)范圍為0.78 ng/mL-50 ng/mL，該產(chǎn)品廣泛應(yīng)用于科研領(lǐng)域，如腸道炎癥性疾病（如克羅恩病、潰瘍性結(jié)腸炎）的機(jī)制研究、細(xì)菌感染模型中宿主 - 病原體相互作用評(píng)估，以及腸道微生物群與免疫調(diào)節(jié)相關(guān)性等基礎(chǔ)研究，為探索鞭毛蛋白在先天免疫中的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

flagellin
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Microbiology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human flagellin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-95
1:2	Average %	95
	Range %	91-99
1:4	Average %	95
	Range %	88-102
1:8	Average %	98
	Range %	91-104

回收率：

The recovery of human flagellin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-93
EDTA plasma (n=4)	95	92-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.