Human fetal Fibronectin(fFN)ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人胎兒纖連蛋白(fFN)酶聯免疫試劑盒（CSB-E17582h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織培養上清液樣本中的fFN含量。fFN（胎兒纖維連接蛋白）是一種細胞外基質糖蛋白，主要分布在羊膜母胎面，絨毛膜和蛻膜之間。在正常情況下，fFN在宮頸陰道分泌物中的水平非常低，但在妊娠22周或之后，fFN水平升高與自發早產風險增加相關聯。fFN檢測可用于預測早產風險，其研究機制主要涉及fFN在妊娠和子宮之間的“黏合劑”作用，以及其在宮頸分泌物中的水平變化。試劑盒檢測范圍為25 ng/mL-1600 ng/mL，該產品適用于生殖醫學領域的基礎研究，例如妊娠相關生物標志物的體外分析、胎兒發育機制探索、病理妊娠模型的蛋白表達水平評估等科研場景，也可用于體外藥物篩選或細胞模型中fFN調控機制的實驗研究，為探索妊娠期分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

fFN
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, cell culture supernates
檢測范圍：

25 ng/mL-1600 ng/mL
靈敏度：

6.25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Human fFN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	92
	Range %	84-96
1:4	Average %	94
	Range %	85-98
1:8	Average %	102
	Range %	98-105

回收率：

The recovery of Human fFN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-96
EDTA plasma (n=4)	89	82-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.