Human estrogen,E ELISA Kit
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中文名稱：人雌激素(E)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E07286h
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人雌激素(E)酶聯(lián)免疫試劑盒（CSB-E07286h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的E含量。試劑盒檢測(cè)范圍為25 pg/mL-1000 pg/mL，該產(chǎn)品可廣泛應(yīng)用于基礎(chǔ)醫(yī)學(xué)研究中激素動(dòng)態(tài)監(jiān)測(cè)、生殖生物學(xué)機(jī)制探索、環(huán)境內(nèi)分泌干擾物評(píng)估，以及藥物開(kāi)發(fā)過(guò)程中雌激素相關(guān)代謝通路的體外分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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別名：N/A
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縮寫：E
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma
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檢測(cè)范圍：25 pg/mL-1000 pg/mL
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靈敏度：25 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Signal Transduction
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測(cè)定原理：quantitative
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測(cè)定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human estrogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 88-101 1:2 Average % 94 Range % 86-100 1:4 Average % 97 Range % 94-101 1:8 Average % 91 Range % 85-96 -
回收率：
The recovery of human estrogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 87-94 EDTA plasma (n=4) 96 92-100 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 0 2.391 2.351 2.371 25 1.542 1.539 1.541 75 0.865 0.842 0.854 230 0.567 0.565 0.566 500 0.361 0.359 0.360 1000 0.156 0.153 0.155 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis L Dong, H Wu, F Qi, W Chen, Y Xu, M Li,Journal of Ovarian Research,2025
- lncRNA NEAT1 affects granulosa cell apoptosis and steroid biosynthesis via the miR-204-5p/ESR1 axis in ovarian aging L Dong, H Wu, F Qi, W Chen, Y Xu, M Li, Y Wang,Research Square,2024
- Protective effect of afamin protein against oxidative stress related injury in human ovarian granulosa cells Q Zhang, X Zheng, X Zhang, L Zheng,Journal of Ovarian Research,2024
- Stearoyl-CoA Desaturases1 Accelerates Non-Small Cell Lung Cancer Metastasis by Promoting Aromatase Expression to Improve Estrogen Synthesis J Chen,International journal of molecular sciences,2023
- Transcriptome Reveals Granulosa Cells Coping through Redox, Inflammatory and Metabolic Mechanisms under Acute Heat Stress A Sammad,Cells,2022
- Joint Transcriptome and Metabolome Analysis Prevails Biological Mechanisms Underlying the Prosurvival Fight in Heat Stressed Granulosa Cells AS Sammad,molecular biology,2022
- The function of BAP18 on modulation of androgen receptor action in luteinized granulosa cells from normal weight women with and without PCOS Yue Zhao,Molecular And Cellular Endocrinology,2021
- Genistein protects ovarian granulosa cells from oxidative stress via cAMP-PKA signaling Luo M, et al,Cell Biology International,2019
- Chronic Estradiol Administration During the Early Stage of Alzheimer's Disease Pathology Rescues Adult Hippocampal Neurogenesis and Ameliorates Cognitive Deficits in Aβ1-42 Mice. J Zheng.et al,Molecular Neurobiology,2016
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