Human estrogen,E ELISA Kit

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中文名稱：

人雌激素(E)酶聯免疫試劑盒
貨號：

CSB-E07286h
規格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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產品描述：

人雌激素(E)酶聯免疫試劑盒（CSB-E07286h）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的E含量。試劑盒檢測范圍為25 pg/mL-1000 pg/mL，該產品可廣泛應用于基礎醫學研究中激素動態監測、生殖生物學機制探索、環境內分泌干擾物評估，以及藥物開發過程中雌激素相關代謝通路的體外分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

E
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

25 pg/mL-1000 pg/mL
靈敏度：

25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human estrogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-101
1:2	Average %	94
	Range %	86-100
1:4	Average %	97
	Range %	94-101
1:8	Average %	91
	Range %	85-96

回收率：

The recovery of human estrogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	96	92-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
0	2.391	2.351	2.371
25	1.542	1.539	1.541
75	0.865	0.842	0.854
230	0.567	0.565	0.566
500	0.361	0.359	0.360
1000	0.156	0.153	0.155

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis L Dong, H Wu, F Qi, W Chen, Y Xu, M Li,Journal of Ovarian Research,2025
lncRNA NEAT1 affects granulosa cell apoptosis and steroid biosynthesis via the miR-204-5p/ESR1 axis in ovarian aging L Dong, H Wu, F Qi, W Chen, Y Xu, M Li, Y Wang,Research Square,2024
Protective effect of afamin protein against oxidative stress related injury in human ovarian granulosa cells Q Zhang, X Zheng, X Zhang, L Zheng,Journal of Ovarian Research,2024
Stearoyl-CoA Desaturases1 Accelerates Non-Small Cell Lung Cancer Metastasis by Promoting Aromatase Expression to Improve Estrogen Synthesis J Chen,International journal of molecular sciences,2023
Transcriptome Reveals Granulosa Cells Coping through Redox, Inflammatory and Metabolic Mechanisms under Acute Heat Stress A Sammad,Cells,2022
Joint Transcriptome and Metabolome Analysis Prevails Biological Mechanisms Underlying the Prosurvival Fight in Heat Stressed Granulosa Cells AS Sammad,molecular biology,2022
The function of BAP18 on modulation of androgen receptor action in luteinized granulosa cells from normal weight women with and without PCOS Yue Zhao,Molecular And Cellular Endocrinology,2021
Genistein protects ovarian granulosa cells from oxidative stress via cAMP-PKA signaling Luo M, et al,Cell Biology International,2019
Chronic Estradiol Administration During the Early Stage of Alzheimer's Disease Pathology Rescues Adult Hippocampal Neurogenesis and Ameliorates Cognitive Deficits in Aβ1-42 Mice. J Zheng.et al,Molecular Neurobiology,2016

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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