Human early prostate cancer antigen 2 (EPCA2) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<20%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人早期前列腺癌抗原2(EPCA2)酶聯(lián)免疫試劑盒（CSB-EQ027679HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EPCA2含量。EPCA2是早期前列腺癌的潛在標(biāo)志物，其研究機(jī)制涉及DNA低甲基化導(dǎo)致抗腫瘤免疫基因沉默，從而促進(jìn)腫瘤免疫逃逸。EPCA2的表達(dá)與前列腺癌的早期發(fā)生和進(jìn)展密切相關(guān)，為腫瘤的早期檢測和治療提供了新的思路。試劑盒檢測范圍為12.5 ng/mL-400 ng/mL，可為前列腺癌的早期分子機(jī)制研究及療效評估提供重要依據(jù)；滿足多種科研場景需求，包括體外細(xì)胞模型、動物實(shí)驗(yàn)或生物樣本庫中前列腺癌相關(guān)蛋白表達(dá)譜分析；適用于分子生物學(xué)、腫瘤學(xué)等領(lǐng)域的基礎(chǔ)研究，為探索前列腺癌病理機(jī)制及潛在治療靶點(diǎn)提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

EPCA2
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 ng/mL-400 ng/mL
靈敏度：

6.25 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human EPCA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	82-96
1:2	Average %	92
	Range %	87-96
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	87-101

回收率：

The recovery of human EPCA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-103
EDTA plasma (n=4)	99	95-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.