Human early prostate cancer antigen 2 (EPCA2) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<20%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人早期前列腺癌抗原2(EPCA2)酶聯免疫試劑盒（CSB-EQ027679HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EPCA2含量。EPCA2是早期前列腺癌的潛在標志物，其研究機制涉及DNA低甲基化導致抗腫瘤免疫基因沉默，從而促進腫瘤免疫逃逸。EPCA2的表達與前列腺癌的早期發生和進展密切相關，為腫瘤的早期檢測和治療提供了新的思路。試劑盒檢測范圍為12.5 ng/mL-400 ng/mL，可為前列腺癌的早期分子機制研究及療效評估提供重要依據；滿足多種科研場景需求，包括體外細胞模型、動物實驗或生物樣本庫中前列腺癌相關蛋白表達譜分析；適用于分子生物學、腫瘤學等領域的基礎研究，為探索前列腺癌病理機制及潛在治療靶點提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

EPCA2
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 ng/mL-400 ng/mL
靈敏度：

6.25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human EPCA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	82-96
1:2	Average %	92
	Range %	87-96
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	87-101

回收率：

The recovery of human EPCA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-103
EDTA plasma (n=4)	99	95-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.