Human dynorphin,Dyn ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人強啡肽(Dyn)酶聯免疫試劑盒（CSB-E09128h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Dyn含量。人強啡肽（Dyn）是一種具有阿片樣活性的內源性肽，主要作用于κ-阿片受體，具有鎮痛、減輕腦水腫、改善缺血后腦記憶障礙等腦保護作用。其作用機制主要通過阿片樣受體途徑和非阿片樣受體途徑實現，如κ受體介導的鎮痛和NMDA受體途徑的腦保護作用。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，本試劑盒檢測范圍為3.12 pg/mL至200 pg/mL，可精準識別低豐度靶標，適用于神經生物學、疼痛機制研究或精神疾病動物模型中的動態監測。操作簡便，僅需100 μL樣本即可完成檢測，配套提供標準品、稀釋液及終止液等完整組分，適用于實驗室規模的高通量篩查或靶向性研究，為探索Dyn在神經信號傳導、藥物干預效果評估等科研領域提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

Dyn
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Dyn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-90
1:2	Average %	95
	Range %	90-100
1:4	Average %	93
	Range %	90-96
1:8	Average %	92
	Range %	87-96

回收率：

The recovery of human Dyn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	84-99
EDTA plasma (n=4)	100	97-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.