Human blca-4 ELISA Kit

產(chǎn)品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人blca-4酶聯(lián)免疫試劑盒（CSB-E14959h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的BLCA-4含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于科研領(lǐng)域中對膀胱癌發(fā)病機制、腫瘤微環(huán)境調(diào)控及生物標志物篩查的研究，支持多種生物樣本類型的平行檢測，為體外實驗提供穩(wěn)定可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

BLCA-4
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human blca-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-91
1:2	Average %	99
	Range %	96-103
1:4	Average %	104
	Range %	101-107
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of human blca-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	84-90
EDTA plasma (n=4)	92	88-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.