Human basic fibroblast growth factor,bFGF ELISA Kit
產品詳情
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產品描述：
人堿性成纖維細胞生長因子(bFGF)酶聯免疫試劑盒（CSB-E08000h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGF2含量。FGF2即成纖維細胞生長因子2，是一種多功能細胞因子。它在胚胎發育、血管生成、組織修復等生理過程中發揮重要作用。研究多聚焦于其信號通路，如與受體結合激活下游信號分子，影響細胞增殖、分化等，是生物醫學領域頗具潛力的研究靶點。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于體外培養細胞上清、動物模型組織或體液樣本的檢測；科研人員可通過該產品探究bFGF在細胞信號通路調控、組織再生、腫瘤微環境中的作用，或用于評估藥物干預、基因編輯等實驗條件下bFGF的表達變化；嚴格適用于生命科學基礎研究，為探索bFGF相關分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：
Basic fibroblast growth factor ELISA Kit; Basic fibroblast growth factor bFGF ELISA Kit; BFGF ELISA Kit; FGF 2 ELISA Kit; FGF B ELISA Kit; FGF-2 ELISA Kit; Fgf2 ELISA Kit; FGF2 basic ELISA Kit; FGF2_HUMAN ELISA Kit; FGFB ELISA Kit; Fibroblast growth factor 2 (basic) ELISA Kit; Fibroblast growth factor 2 ELISA Kit; Fibroblast growth factor; basic ELISA Kit; HBGF 2 ELISA Kit; HBGF-2 ELISA Kit; HBGF2 ELISA Kit; HBGH 2 ELISA Kit; HBGH2 ELISA Kit; Heparin binding growth factor 2 precursor ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; Prostatropin ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, tissue homogenates
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檢測范圍：
1.56 pg/mL-100 pg/mL
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靈敏度：
0.39 pg/mL
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反應時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領域：
Cardiovascular
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
98 |
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| Range % |
93-103 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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回收率：
| The recovery of human bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
85-98 |
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| EDTA plasma (n=4) |
92 |
89-96 |
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標準曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.534 |
2.356 |
2.445 |
2.349 |
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| 50 |
1.961 |
1.957 |
1.959 |
1.863 |
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| 25 |
1.545 |
1.589 |
1.567 |
1.471 |
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| 12.5 |
1.157 |
1.169 |
1.163 |
1.067 |
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| 6.25 |
0.718 |
0.746 |
0.732 |
0.636 |
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| 3.12 |
0.447 |
0.486 |
0.467 |
0.371 |
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| 1.56 |
0.231 |
0.241 |
0.236 |
0.140 |
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| 0 |
0.098 |
0.094 |
0.096 |
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本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human bFGF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled bFGF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數據處理：
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貨期：
3-5 working days
引用文獻
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Air-Dried Human Amniotic Membranes: Sterility, Microbial Barrier, and Cytokine Retention
F Ebrahim, MB Milad, M Saidi, A Elzagheid,Cureus,2025
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Особенности цитокиновой продукции у пациентов с бактериальной пневмонией, протекающей на фоне острой респираторной вирусной инфекции
ВК Парфенюк, НВ Бондарь,Российский медико-биологический вестник имени академика ИП Павлова,2025
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Pretreatment with growth differentiation factor 15 augments cardioprotection by mesenchymal stem cells in myocardial infarction by improving their survival
X Huang, X Liang, Q Han, Y Shen, J Chen, Z Li,Stem Cell Research & Therapy,2024
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Tough and Self-Adhesive Zwitterionic Hydrogels with Mechano-Responsive Release of bFGF for Tympanic Membrane Repair
S Chen, X Guo, Y Yang, J Deng, T Xu, Z Yuan, H Xue,Materials Today Bio,2024
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Enhancing angiogenesis in Peri-Implant soft tissue with bioactive silk fibroin microgroove coatings on zirconia surfaces
Z Wang,Regenerative Biomaterials,2024
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Insufficient S-adenosylhomocysteine hydrolase compromises the beneficial effect of diabetic BMSCs on diabetic cardiomyopathy
Y Wang,Stem cell research & therapy,2022
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Platelet-rich plasma (PRP): an adjuvant to hasten hamstring muscle recovery. A randomized controlled trial protocol (ISCRTN66528592)
/,BMC Musculoskeletal Disorders,2012
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Small RNAs Targeting Transcription Start Site Induce Heparanase Silencing through Interference with Transcription Initiation in Human Cancer Cells
Guosong Jiang et al,PLOS ONE,2012
靶點詳情
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功能：
Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
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基因功能參考文獻：
- The Novel Short Isoform of Securin Stimulates the Expression of Cyclin D3 and Angiogenesis Factors VEGFA and FGF2, but Does Not Affect the Expression of MYC Transcription Factor PMID: 29989583
- miR-155 and FGF2 is associated with esophageal cancer progression. miR-155 in Tumor-associated macrophages suppressed ECA109cell proliferation, migration and invasion, as well as reduction in angiogenesis. miR-155-reduced cell growth, migration and invasion of ECA109cells is associated with FGF2 suppression. PMID: 29660336
- the dual warhead-FGF2 conjugate may overcome the potential acquired resistance of FGFR1-overproducing cancer cells towards single cytotoxic drugs. PMID: 30029518
- widely stained in sclerosing stromal tumours of the ovary PMID: 29433373
- FGF2 initiates CYGB transcription via the JNK pathway. PMID: 28916723
- A strong stromal FGF-2 expression was associated with a significantly higher clinical stage and higher biochemical recurrence rate. Patients with strong stromal FGF-2 expression also had a significantly worse biochemical recurrence-free survival. PMID: 29887238
- levels of the angiogenesis mediators endoglin, HB-EGF, BMP-9 and FGF-2 in patients with severe sepsis and septic shock PMID: 28746898
- Our results suggest photodynamic therapy is effective in increasing the expression of bFGF gene, an important factor in periodontal tissue regeneration and could indicate periodontal tissue regeneration PMID: 28935533
- High FGF2 expression is associated with gastric cancer. PMID: 28500362
- Regulation of vascular smooth muscle cell calcification by syndecan-4/FGF-2/PKCalpha signalling and cross-talk with TGF-beta1. PMID: 29016732
- evaluation of the presence and localization of FGF2 in human sperm cells, and determination of FGF2 levels in semen samples and its relationship with conventional semen parameters PMID: 28732140
- We have presented evidence that FGF2 promotes myofibroblast apoptosis in vivo, antagonizes pro-fibrotic TGF-beta signaling, inhibits fibroblast activation and prevents transdifferentiation of non-fibroblasts into myofibroblasts, and promotes a less fibrotic gene expression paradigm [Review] PMID: 28967471
- These results demonstrate that FGF-TGFbeta signaling antagonism is the primary regulator of the smooth muscle cell phenotype switch. PMID: 27634335
- Results describe a novel role of FGF2 as a modulator of osteoblast and mesenchymal stromal cell function, and provide evidence for involvement of FGF2 in leukemia pathogenesis and chemo-resistance. PMID: 27481339
- Under specific experimental conditions, secretion of IL-1beta and FGF2 is triggered by phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2]-dependent formation of pores across the plasma membrane. PMID: 28871048
- HDAC1 depletion activates cardiac mesenchymal stromal cells proangiogenic paracrine signaling in a basic fibroblast growth factor-dependent manner. PMID: 28679560
- The results suggest that FGF2/rs1048201, FGF5/rs3733336 and FGF9/rs546782 are associated with the risk of non-syndromic orofacial cleft and that these miRNA-FGF interactions may affect non-syndromic orofacial cleft development. PMID: 27511275
- TEC is yet another regulator of FGF2-mediated Human pluripotent stem cells pluripotency and differentiation. PMID: 28631381
- bFGF in primary tumor tissue associated with favorable breast cancer outcome and its levels significantly and positively correlated with ER levels. PMID: 28869446
- Serum FGF-2 levels were statistically significantly lower in the autism spectrum patient group compared to healthy controls. PMID: 28734240
- Dysregulation of the FGF2 gene represents an opportunity to understand further, and possibly intervene upon, mechanisms of wound healing in diabetics with CKD. PMID: 27237708
- Out of five FGF-2 Gene Polymorphism loci, the TA genotype of rs308442 in the osteoporosis group (40.2%) was higher than in the control group (29.5%) (p < 0.05). The rs308442 locus of FGF-2 gene is closely correlated to osteoporosis in this Zhuang ethnic Chinese cohort, and the TA may be the risk genotype of osteoporosis. PMID: 28653999
- Up-regulation of FGF2 and down-regulation FAM201A were correlated with the development of osteonecrosis of the femoral head after femoral neck fracture. PMID: 29382571
- over-expression, isolation, and biological activity of all recombinant human FGF2 isoforms, are reported. PMID: 28433654
- We found that hsa-miR-196a-3p affected expression on both mRNA and protein levels of FGF2. our study provided evidence that a functional SNP rs1048201 was associated with bone mineral density (BMD), and SNP rs1048201 variant may act by affecting binding of hsa-miR-196a-3p PMID: 28317323
- Data show that mutant soluble ectodomain of FGFR2IIIc (msFGFR2c) but not wild-type soluble ectodomain of FGFR2IIIc (wsFGFR2c) could selectively bind to c subtype of FGFRs in the presence of FGF-2. PMID: 28049184
- Furthermore, MERS coronavirus induced apoptosis through upregulation of Smad7 and fibroblast growth factor 2 (FGF2) expression in both kidney and lung cells. PMID: 27572168
- The present work aims to investigate the relationship between the expression of AEG-1(astrocyte elevated gene-1), b-FGF(basic-fibroblast growth factor), beta-catenin, Ki-67, TNF-alpha (tumor necrosis factor-alfa) other prognostic parameters in DC (Ductal Carcinomas) and ductal intraepithelial neoplasm. We found a relationship between these factors. PMID: 26096243
- Studies indicate that therapeutically targeting FGF2 and FGFR has been extensively assessed in multiple preclinical studies and numerous drugs and treatment options have been tested in clinical trials. PMID: 27007053
- FGF2 is involved in melanoma development and progression. HMW FGF2 isoforms enhance in vitro motility of melanoma cells. LMW FGF2 confers stem-like features and increases in vivo metastasization. LMW FGF2 promotes angiogenesis while HMW FGF2 induces vasculogenic mimicry. PMID: 27558498
- miR-105/Runx2 axis mediates FGF2-induced ADAMTS expression in osteoarthritis cartilage. PMID: 26816250
- results suggest that MALAT1-mediated FGF2 protein secretion from Tumor-associated macrophages inhibits inflammatory cytokines release, promotes proliferation, migration, and invasion of FTC133 cells and induces vasculature formation. PMID: 28543663
- This study reveals that Adv ECM hydrogels recapitulate matrix fiber microarchitecture of native adventitia, and retain angiogenesis-related actors and bioactive properties such as FGF2 signaling capable of influencing processes important for angiogenesis. PMID: 28167392
- Data show that FGF2 mutants have potential as anti-angiogenic agents and useful tools for studying the role of integrin alphavbeta3 in FGF2 signalling. PMID: 28302677
- Expression of these mediators was confirmed in end-stage COPD. Thus, accumulation of mast cells in COPD may contribute to vascular remodeling. PMID: 28298222
- Results provide evidence that bFGF regulates stemness maintenance in stem cells isolated from human exfoliated deciduous teeth (SHEDs) by enhancing REX-1 mRNA expression via the FGFR and Akt signaling pathways. Moreover, IL-6 is also involved in the bFGF-induced REX1 expression. PMID: 27883224
- Facial nerve regeneration using basic fibroblast growth factor-impregnated gelatin microspheres PMID: 24737684
- These results indicated that FGF-2, but not FGF-10, may be supplemented during stem cell expansion to prime cells for successful chondrogenesis and osteogenesis. PMID: 27411850
- the data suggest that endothelial cells regulate beta-catenin activity in adrenocortical cells also via secretion of basic fibroblast growth factor. PMID: 27889473
- In an in vitro assay of vascular smooth muscle cells, circRNA WDR77 silencing significantly inhibited cell proliferation and migration. Bioinformatics methods revealed that miR-124 and fibroblast growth factor 2 (FGF-2) were downstream targets of circRNA WDR77. PMID: 29042195
- FGF2 protects the tumor cells from the antiproliferative effect of Gefitinib and largely prevents reprogramming of the proteome and phosphoproteome PMID: 27794612
- High FGF2 expression is associated with colon cancer metastasis. PMID: 28629469
- These observations identify airway smooth muscle cells -derived FGF2b as a factor needed for LMC formation by CD4 T cells, affecting intercellular communication. PMID: 28924004
- Report no relationship between serum bFGF levels and ovarian cancer microvessel density and tumor bFGF expression. PMID: 27312585
- High FGF2 expression is associated with ovarian cancer. PMID: 28481872
- The antitumor activity of scopoletin may be due to its strong anti-angiogenic effect, which may be mediated by its effective inhibition of ERK1, VEGF-A, and FGF-2. PMID: 27133199
- TGF-beta, bFGF and epimorphin in the extracellular microenvironment cooperatively affect HSF behaviors under the control of a highly sulfated chondroitin sulfate PMID: 28209294
- High FGF2 expression is associated with lung cancer. PMID: 28423625
- miR-205 enhances chemosensitivity of breast cancer cells to TAC docetaxol, doxorubicin plus cyclophosphamide) by suppressing both VEGFA and FGF2, leading to evasion of apoptosis. PMID: 27362808
- analyses identified a new bFGF-regulating mechanism by which hedgehog signaling regulates human fibroblast migration; this data opens a new avenue for the wound healing therapy PMID: 28363830
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亞細胞定位：
Secreted. Nucleus.
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蛋白家族：
Heparin-binding growth factors family
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組織特異性：
Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
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數據庫鏈接：
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