Human anti-Survivin(Surv) antibody ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人存活素(Surv)抗體酶聯(lián)免疫試劑盒（CSB-E11874h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的Surv Ab含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于探索該靶點在細胞凋亡調(diào)控與腫瘤發(fā)生機制中的科研應用。科研人員可將其用于體外培養(yǎng)細胞上清液、實驗動物模型血清或臨床前研究血漿樣本中Survivin蛋白表達水平的動態(tài)監(jiān)測，為腫瘤發(fā)生機制解析、藥物敏感性評估及治療方案優(yōu)化提供數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Surv Ab
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human anti-surv antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-90
1:2	Average %	96
	Range %	90-105
1:4	Average %	86
	Range %	81-90
1:8	Average %	93
	Range %	85-100

回收率：

The recovery of human anti-surv antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-101
EDTA plasma (n=4)	87	82-92

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.