Human alpha II-spectrin breakdown product SBDP145 ELISA kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人αII-血影蛋白分解產物SBDP145酶聯(lián)免疫試劑盒（CSB-EQ028022HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、腦脊液（CSF）樣本中的SBDP145含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，在科研領域，廣泛應用于創(chuàng)傷性腦損傷模型評估、神經退行性疾病機制研究、細胞凋亡信號通路分析等方向，尤其適用于腦脊液樣本中神經損傷標志物的動態(tài)監(jiān)測，以及通過組織勻漿分析局部損傷程度，為神經病理學、藥理學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
縮寫：

SBDP145
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SBDP145 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	100
	Range %	95-105
1:4	Average %	90
	Range %	85-96
1:8	Average %	105
	Range %	100-109

回收率：

The recovery of human SBDP145 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	85-90
EDTA plasma (n=4)	90	85-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.