Human alcohol dehydrogenase,ADH ELISA Kit

Human alcohol dehydrogenase,ADH ELISA Kit

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中文名稱：

人乙醇脫氫酶(ADH)酶聯免疫試劑盒
貨號：

CSB-E11855h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人乙醇脫氫酶(ADH)酶聯免疫試劑盒（CSB-E11855h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿、細胞裂解物樣本中的ADH1A含量。ADH1A是一個重要靶點。它在乙醇代謝等生理過程中發揮關鍵作用。研究機制方面，聚焦于其基因表達調控及蛋白功能。通過探究它與疾病的關聯，如酒精性肝病等，為開發針對性治療藥物和干預策略提供理論依據和研究方向。試劑盒檢測范圍為0.312 mU/mL-20 mU/mL，適用于酒精代謝機制研究、肝損傷模型評估、藥物或化合物對ADH活性調控作用分析等科研場景，特別針對體外培養細胞或動物實驗樣本中的ADH活性檢測需求開發，為代謝組學、毒理學及肝臟病理研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

ADH alpha subunit ELISA Kit; ADH ELISA Kit; ADH1 ELISA Kit; ADH1A ELISA Kit; ADH1A_HUMAN ELISA Kit; Alcohol dehydrogenase 1 (class I), alpha polypeptide ELISA Kit; Alcohol dehydrogenase 1 ELISA Kit; Alcohol dehydrogenase 1A (class I), alpha polypeptide ELISA Kit; Alcohol dehydrogenase 1A ELISA Kit; Alcohol dehydrogenase subunit alpha ELISA Kit; Aldehyde reductase ELISA Kit
縮寫：

ADH1A
Uniprot No.：

P07327
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
檢測范圍：

0.312 mU/mL-20 mU/mL
靈敏度：

0.078 mU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-87
1:2	Average %	102
	Range %	97-107
1:4	Average %	94
	Range %	90-99
1:8	Average %	86
	Range %	83-89

回收率：

The recovery of human ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	99-105
EDTA plasma (n=4)	97	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
20	2.294	2.156	2.225	2.107
10	1.778	1.698	1.738	1.620
5	1.253	1.240	1.247	1.129
2.5	0.797	0.775	0.786	0.668
1.25	0.485	0.468	0.477	0.359
0.625	0.362	0.375	0.369	0.251
0.312	0.256	0.262	0.259	0.141
0	0.119	0.117	0.118

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

基因功能參考文獻：
1. High ADH expression is associated with Prostate Cancer. PMID: 28870918
2. Results suggest a potential role of alcohol dehydrogenase (ADH) class I as a marker for renal cell carcinoma (RCC). PMID: 27086037
3. Data indicate that the up-regulation of aldehyde dehydrogenase-1 (ALDH1) after neoadjuvant chemotherapy (NAC) predicts poor survival in locally advanced breast cancer. PMID: 24762066
4. Unde-rexpression of the ADH1 gene, which influences the transformation of the extracellular matrix, plays a probable role in the etiology of uterine fibroid. PMID: 23891545
5. high levels of ADH1 transcription are implicated in fluconazole resistance in C. albicans and that the mRNA expression levels of ADH1 are positively correlated with those of CDR1, CDR2 and FLU1. PMID: 23769565
6. common ADH variants conferred risk for both schizophrenia in African-Americans and autism in European-Americans. PMID: 23468174
7. High ADH1 is associated with endometrial cancer. PMID: 20872569
8. ADH1A variation predisposes to personality traits and substance dependence PMID: 19526455
9. Structural aspects that influence dimer-tetramer formation. PMID: 12081471
10. GATA-2 and HNF-3beta regulate the human alcohol dehydrogenase 1A (ADH1A) gene. PMID: 16153155
11. The oxidative pathway of ethanol metabolism via ADH and ALDH does not play a role in pancreatic carcinogenesis. PMID: 17632320
12. Alleles of ADH7 SNPs were associated with the early stages of alcohol metabolism, with additional effects in the ADH1A, ADH1B and ADH4 regions. PMID: 19193628
13. Observational study of healthcare-related. (HuGE Navigator) PMID: 15279067
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亞細胞定位：

Cytoplasm.
蛋白家族：

Zinc-containing alcohol dehydrogenase family
數據庫鏈接：

HGNC: 249

OMIM: 103700

KEGG: hsa:124

STRING: 9606.ENSP00000209668

UniGene: Hs.654433

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.