Human activated coagulation factor Ⅻ,FⅫa ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人活化凝血因子Ⅻ(FⅫa)酶聯(lián)免疫試劑盒（CSB-E09232h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FⅫa含量。FⅫa是凝血因子Ⅻ激活后形成的絲氨酸蛋白酶。其參與內(nèi)源性凝血途徑啟動。研究發(fā)現(xiàn)它不僅在凝血中起作用，還與炎癥、血栓形成等相關(guān)。對FⅫa作用機(jī)制的研究，有助于開發(fā)新的抗血栓藥物，且可能減少出血風(fēng)險。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，本試劑盒通過高特異性抗體對靶標(biāo)進(jìn)行捕獲與檢測，適用于體外研究FⅫa在凝血機(jī)制、血管通透性調(diào)控或相關(guān)疾病動物模型中的動態(tài)變化，可為心血管疾病、免疫炎癥反應(yīng)等領(lǐng)域的科研實(shí)驗(yàn)提供定量分析支持。該產(chǎn)品僅用于科學(xué)研究，不適用于其他用途。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

FⅫa
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FⅫa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	111
	Range %	105-116
1:2	Average %	95
	Range %	88-102
1:4	Average %	96
	Range %	90-100
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human FⅫa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-99
EDTA plasma (n=4)	108	103-114

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.