Human Xin actin-binding repeat-containing protein 1(XIRP1) ELISA kit

Human Xin actin-binding repeat-containing protein 1(XIRP1) ELISA kit

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中文名稱：

人Xin肌動(dòng)蛋白結(jié)合含重復(fù)序列蛋白(XIRP1)酶聯(lián)免疫試劑盒
貨號：

CSB-EL026195HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人Xin肌動(dòng)蛋白結(jié)合含重復(fù)序列蛋白(XIRP1)酶聯(lián)免疫試劑盒（CSB-EL026195HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的XIRP1含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，該產(chǎn)品適用于基礎(chǔ)科研中XIRP1的功能機(jī)制研究，例如心血管疾病模型中的蛋白表達(dá)動(dòng)態(tài)分析、腫瘤微環(huán)境下的細(xì)胞遷移能力評估，或藥物干預(yù)實(shí)驗(yàn)中分子標(biāo)記物的定量追蹤，為體外實(shí)驗(yàn)提供可靠的蛋白水平檢測方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

AI415219 ELISA Kit; Cardiomyopathy-associated protein 1 ELISA Kit; CMYA1 ELISA Kit; DKFZp451D042 ELISA Kit; DKFZp779C1255 ELISA Kit; DKFZp779C1947 ELISA Kit; MGC144145 ELISA Kit; MGC144146 ELISA Kit; Xin actin-binding repeat-containing protein 1 ELISA Kit; XIN ELISA Kit; Xirp1 ELISA Kit; XIRP1_HUMAN ELISA Kit
縮寫：

XIRP1
Uniprot No.：

Q702N8
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human XIRP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	90
	Range %	84-95
1:4	Average %	99
	Range %	95-106
1:8	Average %	97
	Range %	90-104

回收率：

The recovery of human XIRP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	93-96
EDTA plasma (n=4)	101	98-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	1.917	1.947	1.932	1.750
1000	1.286	1.299	1.293	1.111
500	0.804	0.815	0.810	0.628
250	0.536	0.505	0.521	0.339
125	0.401	0.435	0.418	0.236
62.5	0.312	0.323	0.318	0.136
31.25	0.247	0.261	0.254	0.072
0	0.181	0.183	0.182

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Protects actin filaments from depolymerization.
基因功能參考文獻(xiàn)：
1. Abnormal CMYA1 expression affects the phosphorylation of Cx43 through the protein kinase c signaling pathway, which is involved in the regulation of gap junction intercellular communication. PMID: 29176328
2. Aciculin interacts with filamin C and Xin and is essential for myofibril assembly. PMID: 24963132
3. The strong correlation between the degree of muscle damage and Xin immunoreactivity suggests that Xin may be a suitable outcome measure to evaluate disease progression and treatment effects in clinical trials. PMID: 24225086
4. We identify the SH3 domains of nebulin and nebulette as novel ligands of proline-rich regions of Xin and XIRP2. PMID: 23985323
5. Data provide further support that ALS2CL, EPHA3, and CMYA1 are bona-fide tumor-suppressor genes and contribute to the tumorigenesis of HNSCC. PMID: 20657180
6. Here, we identify Xin, the protein encoded by the human gene 'cardiomyopathy associated 1'(CMYA1) as filamin c binding partner at these specialized structures where the ends of myofibrils are attached to the sarcolemma PMID: 16631741
7. Data show that in myofibrillar myopathies Xin protein exhibites significant alterations in their localization. PMID: 19151983
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亞細(xì)胞定位：

Cell junction. Note=Colocalizes with actin stress fibers.
蛋白家族：

Xin family
組織特異性：

Isoform A, isoform B and isoform C are expressed in heart.
數(shù)據(jù)庫鏈接：

HGNC: 14301

OMIM: 609777

KEGG: hsa:165904

STRING: 9606.ENSP00000343140

UniGene: Hs.447868

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.