Human Vascular Endothelial cell Growth Factor B,VEGF-B ELISA KIT

Human Vascular Endothelial cell Growth Factor B,VEGF-B ELISA KIT

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中文名稱：

人血管內皮細胞生長因子B(VEGF-B)酶聯免疫試劑盒
貨號：

CSB-E04758h
規格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人血管內皮細胞生長因子B(VEGF-B)酶聯免疫試劑盒（CSB-E04758h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VEGFB含量。VEGFB是血管內皮生長因子家族成員。其背景上，在血管生成等生理過程中有一定關聯。研究機制方面，它可通過與受體結合，調控內皮細胞的活動，在血管發育、維持血管穩態等方面發揮作用，目前也是血管相關疾病研究的潛在靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，廣泛應用于心血管疾病機制研究、代謝相關疾病模型評估、神經退行性病變干預實驗等科研場景，尤其適用于探究VEGF - B在組織修復、能量代謝調控及疾病微環境中的作用機制，為體外實驗提供可靠的蛋白定量解決方案。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Vascular endothelial growth factor B ELISA Kit; Vascular endothelial growth factor-related factor ELISA Kit; VEGF B ELISA Kit; VEGF related factor ELISA Kit; VEGF-B ELISA Kit; VEGF-related factor ELISA Kit; Vegfb ELISA Kit; VEGFB_HUMAN ELISA Kit; VEGFL ELISA Kit; VRF ELISA Kit
縮寫：

VEGFB
Uniprot No.：

P49765
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-96
1:2	Average %	100
	Range %	95-104
1:4	Average %	99
	Range %	92-110
1:8	Average %	89
	Range %	86-96

回收率：

The recovery of human VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-105
EDTA plasma (n=4)	96	92-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.697	2.512	2.605	2.463
500	2.322	2.201	2.262	2.120
250	1.546	1.498	1.522	1.380
125	0.815	0.789	0.802	0.660
62.5	0.417	0.410	0.414	0.272
31.2	0.281	0.276	0.279	0.137
15.6	0.219	0.213	0.216	0.074
0	0.143	0.140	0.142

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

The Aryl Hydrocarbon Receptor–Dependent TGF-α/VEGF-B Ratio Correlates With Disease Subtype and Prognosis in Multiple Sclerosis A Cirac,Neurol Neuroimmunol Neuroinflamm,2021

功能：

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
基因功能參考文獻：
1. High plasma VEGF-B levels are associated with type 2 diabetes mellitus. PMID: 28523459
2. Data from clinical studies point out the changes in circulating or tissue expression levels of VEGF-B in obese compared with lean patients. PMID: 28798193
3. Cardiac transgenic vascular endothelial growth factor-B overexpression failed to protect heart transplants from ischemia-reperfusion injury. PMID: 27588416
4. renal VEGF-B expression correlates with the severity of Diabetic Kidney Disease. PMID: 28190774
5. Data show that metformin treatment reduces serum vascular endothelial growth factor B (VEGF-B) levels and ameliorates insulin resistance. PMID: 26387747
6. Frameshift mutations of VEGFB gene is associated with stomach and colorectal cancers. PMID: 25633991
7. fluid shear stress induces the synthesis of Insulin growth factor-2 and vascular endothelial growth factor (VEGF) B and D, which in turn transactivate MMP-12. PMID: 25435370
8. MMP9 may activate VEGF-B via PI3K/Akt signaling pathway. PMID: 25424698
9. Roles of vascular endothelial growth factor in amyotrophic lateral sclerosis. PMID: 24987705
10. Low VEGFB and VEGFD gene expression is associated with early-stage non-small cell lung cancer. PMID: 24145997
11. Our study suggested that VEGF-B was an angiogenesis factor in vitro and that ERK1/2 and p38-related signaling pathways were involved in these VEGF-B activities. PMID: 24374930
12. VEGF-B has possible roles in cardiac protection, energy metabolism support, and neuroprotectin [review] PMID: 24987005
13. High VEGF-B levels might correlate with the presence of hyperlipidemia and target organ damage in type 2 diabetic patients. PMID: 25001655
14. High VEGFB expression is associated with bone marrow metastasis in neuroblastoma. PMID: 23553333
15. VEGF-B might be an important ligand in the signalling between the tumor and preexisting blood vessels to ensure a functional blood supply for tumor survival. PMID: 23417498
16. we report significant associations with overall survival and distant failure for certain VEGF(VEGF-B) family members. PMID: 23728940
17. Data indicate that three miRs (miR-484, -642, and -217) were able to predict chemoresistance and vasculature of serous epithelial ovarian carcinomas through the regulation of the VEGFB and VEGFR2 pathways. PMID: 23697367
18. Expression of VEGF-B genes in glioma cell lines U87 is significantly changed under hypoxia and ischemic conditions. PMID: 23350126
19. In WT1 mutant cells, reduced VEGF(165)b was due to lack of WT1-mediated transcriptional repression of the splicing-factor kinase SRPK1 PMID: 22172722
20. analysis of binding of vascular endothelial growth factor-B by VEGFR-1(D2) PMID: 20501651
21. TIMP3 blocks the binding of VEGF to VEGF receptor-2 and inhibits downstream signaling and angiogenesis. PMID: 12652295
22. Results describe the crystal structure of human vascular endothelial growth factor-B (VEGF-B) and present a predicted model for the association of VEGF-B with the second domain of its receptor, VEGFR-1. PMID: 16616187
23. Basophils could play a role in angiogenesis and inflammation through the expression of several forms of VEGF-B and their receptors. PMID: 17082651
24. VEGFB, and receptor were highly expressed in dysplastic neurons. IR in astroglial and balloon cells was observed for VEGFA and its receptors Double-labeling also showed expression of VEGFA, VEGFB and VEGFR-1 in cells of the microglia/macrophage lineage. PMID: 18317782
25. VEGF-B appears to have a relatively restricted angiogenic activity in the ischemic heart. PMID: 18511699
26. Increased VEGFB expression is associated with hepatocellular carcinoma PMID: 18537151
27. Overexpression of vascular endothelial growth factor-B in mouse heart alters cardiac lipid metabolism and induces myocardial hypertrophy. PMID: 18757827
28. The structural features of the 'highly ordered' interaction of the Fab fragment of the antibody (Fab-2H10) with VEGF-B, is presented. PMID: 18930733
29. VEGF-B mRNA was not expressed either in normal urothelium or in bladder cancer. PMID: 19424629
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亞細胞定位：

Secreted. Note=Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
蛋白家族：

PDGF/VEGF growth factor family
組織特異性：

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
數據庫鏈接：

HGNC: 12681

OMIM: 601398

KEGG: hsa:7423

STRING: 9606.ENSP00000311127

UniGene: Hs.732095

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.