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中文名稱：人血管內(nèi)皮細胞生長因子B(VEGF-B)酶聯(lián)免疫試劑盒
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貨號：CSB-E04758h
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規(guī)格：96T/48T
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價格：￥3200/￥2500
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促銷：
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人血管內(nèi)皮細胞生長因子B(VEGF-B)酶聯(lián)免疫試劑盒（CSB-E04758h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VEGFB含量。VEGFB是血管內(nèi)皮生長因子家族成員。其背景上，在血管生成等生理過程中有一定關(guān)聯(lián)。研究機制方面，它可通過與受體結(jié)合，調(diào)控內(nèi)皮細胞的活動，在血管發(fā)育、維持血管穩(wěn)態(tài)等方面發(fā)揮作用，目前也是血管相關(guān)疾病研究的潛在靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，廣泛應用于心血管疾病機制研究、代謝相關(guān)疾病模型評估、神經(jīng)退行性病變干預實驗等科研場景，尤其適用于探究VEGF - B在組織修復、能量代謝調(diào)控及疾病微環(huán)境中的作用機制，為體外實驗提供可靠的蛋白定量解決方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Vascular endothelial growth factor B ELISA Kit; Vascular endothelial growth factor-related factor ELISA Kit; VEGF B ELISA Kit; VEGF related factor ELISA Kit; VEGF-B ELISA Kit; VEGF-related factor ELISA Kit; Vegfb ELISA Kit; VEGFB_HUMAN ELISA Kit; VEGFL ELISA Kit; VRF ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：15.6 pg/mL-1000 pg/mL
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靈敏度：3.9 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Cancer
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 88 Range % 80-96 1:2 Average % 100 Range % 95-104 1:4 Average % 99 Range % 92-110 1:8 Average % 89 Range % 86-96 -
回收率：
The recovery of human VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 99 95-105 EDTA plasma (n=4) 96 92-100 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1000 2.697 2.512 2.605 2.463 500 2.322 2.201 2.262 2.120 250 1.546 1.498 1.522 1.380 125 0.815 0.789 0.802 0.660 62.5 0.417 0.410 0.414 0.272 31.2 0.281 0.276 0.279 0.137 15.6 0.219 0.213 0.216 0.074 0 0.143 0.140 0.142 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻
- The Aryl Hydrocarbon Receptor–Dependent TGF-α/VEGF-B Ratio Correlates With Disease Subtype and Prognosis in Multiple Sclerosis A Cirac,Neurol Neuroimmunol Neuroinflamm,2021
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
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基因功能參考文獻：
- High plasma VEGF-B levels are associated with type 2 diabetes mellitus. PMID: 28523459
- Data from clinical studies point out the changes in circulating or tissue expression levels of VEGF-B in obese compared with lean patients. PMID: 28798193
- Cardiac transgenic vascular endothelial growth factor-B overexpression failed to protect heart transplants from ischemia-reperfusion injury. PMID: 27588416
- renal VEGF-B expression correlates with the severity of Diabetic Kidney Disease. PMID: 28190774
- Data show that metformin treatment reduces serum vascular endothelial growth factor B (VEGF-B) levels and ameliorates insulin resistance. PMID: 26387747
- Frameshift mutations of VEGFB gene is associated with stomach and colorectal cancers. PMID: 25633991
- fluid shear stress induces the synthesis of Insulin growth factor-2 and vascular endothelial growth factor (VEGF) B and D, which in turn transactivate MMP-12. PMID: 25435370
- MMP9 may activate VEGF-B via PI3K/Akt signaling pathway. PMID: 25424698
- Roles of vascular endothelial growth factor in amyotrophic lateral sclerosis. PMID: 24987705
- Low VEGFB and VEGFD gene expression is associated with early-stage non-small cell lung cancer. PMID: 24145997
- Our study suggested that VEGF-B was an angiogenesis factor in vitro and that ERK1/2 and p38-related signaling pathways were involved in these VEGF-B activities. PMID: 24374930
- VEGF-B has possible roles in cardiac protection, energy metabolism support, and neuroprotectin [review] PMID: 24987005
- High VEGF-B levels might correlate with the presence of hyperlipidemia and target organ damage in type 2 diabetic patients. PMID: 25001655
- High VEGFB expression is associated with bone marrow metastasis in neuroblastoma. PMID: 23553333
- VEGF-B might be an important ligand in the signalling between the tumor and preexisting blood vessels to ensure a functional blood supply for tumor survival. PMID: 23417498
- we report significant associations with overall survival and distant failure for certain VEGF(VEGF-B) family members. PMID: 23728940
- Data indicate that three miRs (miR-484, -642, and -217) were able to predict chemoresistance and vasculature of serous epithelial ovarian carcinomas through the regulation of the VEGFB and VEGFR2 pathways. PMID: 23697367
- Expression of VEGF-B genes in glioma cell lines U87 is significantly changed under hypoxia and ischemic conditions. PMID: 23350126
- In WT1 mutant cells, reduced VEGF(165)b was due to lack of WT1-mediated transcriptional repression of the splicing-factor kinase SRPK1 PMID: 22172722
- analysis of binding of vascular endothelial growth factor-B by VEGFR-1(D2) PMID: 20501651
- TIMP3 blocks the binding of VEGF to VEGF receptor-2 and inhibits downstream signaling and angiogenesis. PMID: 12652295
- Results describe the crystal structure of human vascular endothelial growth factor-B (VEGF-B) and present a predicted model for the association of VEGF-B with the second domain of its receptor, VEGFR-1. PMID: 16616187
- Basophils could play a role in angiogenesis and inflammation through the expression of several forms of VEGF-B and their receptors. PMID: 17082651
- VEGFB, and receptor were highly expressed in dysplastic neurons. IR in astroglial and balloon cells was observed for VEGFA and its receptors Double-labeling also showed expression of VEGFA, VEGFB and VEGFR-1 in cells of the microglia/macrophage lineage. PMID: 18317782
- VEGF-B appears to have a relatively restricted angiogenic activity in the ischemic heart. PMID: 18511699
- Increased VEGFB expression is associated with hepatocellular carcinoma PMID: 18537151
- Overexpression of vascular endothelial growth factor-B in mouse heart alters cardiac lipid metabolism and induces myocardial hypertrophy. PMID: 18757827
- The structural features of the 'highly ordered' interaction of the Fab fragment of the antibody (Fab-2H10) with VEGF-B, is presented. PMID: 18930733
- VEGF-B mRNA was not expressed either in normal urothelium or in bladder cancer. PMID: 19424629
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亞細胞定位：Secreted. Note=Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
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蛋白家族：PDGF/VEGF growth factor family
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組織特異性：Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
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數(shù)據(jù)庫鏈接：
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