Human Vascular Endothelial Growth Factor 121(VEGF121) ELISA kit

Human Vascular Endothelial Growth Factor 121(VEGF121) ELISA kit

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中文名稱：

人血管內皮生長因子121(VEGF121)酶聯免疫試劑盒
貨號：

CSB-E13709h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人血管內皮生長因子121(VEGF121)酶聯免疫試劑盒（CSB-E13709h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的VEGF121含量。VEGF121 是血管內皮生長因子（VEGF）的一種主要亞型。它在血管生成、腫瘤生長和轉移等過程中起關鍵作用。其研究機制主要聚焦于與受體結合，激活下游信號通路，促進血管內皮細胞的增殖、遷移和存活，目前是腫瘤等疾病治療的重要靶點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，腫瘤血管生成機制研究、心血管疾病模型評估、再生醫學實驗等科研場景，體外實驗、動物模型及細胞水平的研究本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

VEGF121
Uniprot No.：

P15692
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF121 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-106
1:2	Average %	94
	Range %	90-98
1:4	Average %	89
	Range %	86-96
1:8	Average %	92
	Range %	86-97

回收率：

The recovery of human VEGF121 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-104
EDTA plasma (n=4)	91	87-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.415	2.314	2.365	2.222
5	1.532	1.633	1.583	1.440
2.5	0.972	0.951	0.962	0.819
1.25	0.558	0.547	0.553	0.410
0.625	0.404	0.411	0.408	0.265
0.312	0.285	0.274	0.280	0.137
0.156	0.226	0.220	0.223	0.080
0	0.143	0.142	0.143

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.
相關疾病：

Microvascular complications of diabetes 1 (MVCD1)
亞細胞定位：

Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a significant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
蛋白家族：

PDGF/VEGF growth factor family
組織特異性：

Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland.
數據庫鏈接：

HGNC: 12680

OMIM: 192240

KEGG: hsa:7422

STRING: 9606.ENSP00000361125

UniGene: Hs.73793

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.