Human Tryptase beta-2(TPSB2) ELISA kit
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中文名稱：人胰蛋白酶β-2(TPSB2)酶聯免疫試劑盒
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貨號：CSB-EL024128HU
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規格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：人胰蛋白酶β-2(TPSB2)酶聯免疫試劑盒（CSB-EL024128HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TPSB2含量。TPSB2（類胰蛋白酶2）是一種胰蛋白酶樣絲氨酸蛋白酶，屬于肽酶家族S1。它僅作為肝素穩定的四聚體具有酶促活性，對所有已知的內源性蛋白酶抑制劑具有抗性，并在哮喘和其他過敏性和炎癥性疾病發病機制中發揮作用。試劑盒檢測范圍為0.137 ng/ml- 100 ng/ml，支持科研領域對TPSB2的功能研究，例如炎癥性疾病模型的分子機制探索、藥物干預效果評估或細胞分泌功能分析，也可用于生物樣本中TPSB2表達水平的定量比較實驗。適用于生命科學基礎研究及生物標志物開發等場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Mast cell tryptase beta II ELISA Kit; Mast cell tryptase beta III ELISA Kit; TPS2 ELISA Kit; TPSB2 ELISA Kit; TRYB2_HUMAN ELISA Kit; Tryptase beta 2 (gene/pseudogene) ELISA Kit; Tryptase beta-2 ELISA Kit; Tryptase II ELISA Kit; Tryptase-2 ELISA Kit; TryptaseB ELISA Kit; TryptaseC ELISA Kit
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縮寫：TPSB2
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：0.137 ng/ml - 100 ng/ml
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靈敏度：0.137 ng/ml
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Cell Biology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human TPSB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
92
Range %
85-99
1:2
Average %
89
Range %
84-95
1:4
Average %
93
Range %
89-98
1:8
Average %
90
Range %
83-97
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回收率：
The recovery of human TPSB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
93
86-100
EDTA plasma (n=4)
101
96-107
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標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
100
2.576
2.634
2.605
2.502
33.33
1.807
1.767
1.787
1.684
11.11
1.124
1.188
1.156
1.053
3.7
0.639
0.629
0.634
0.531
1.23
0.388
0.371
0.380
0.277
0.41
0.296
0.286
0.291
0.188
0.137
0.185
0.184
0.185
0.082
0
0.101
0.105
0.103
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數據處理：
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貨期：3-5 working days
產品評價
相關產品
靶點詳情
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功能：Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
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基因功能參考文獻：
- These observations indicate that betaII-tryptase activity is post-translationally regulated by an allosteric disulfide bond. PMID: 24142694
- Results indicate a key role for heparin in the activation of human betaI- and beta2-tryptase. PMID: 15567416
- The disease severity and plasma tryptase levels were not affected by the number of alpha or beta tryptase alleles in mastocytosis patients. PMID: 17449330
- Strong linkage of TPSAB1 and TPSB2 and pairing of deficiency alleles with functional alleles in observed haplotypes protect human subjects from "knockout" genomes and indeed from inheritance of fewer than 2 active alleles. PMID: 19748655
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亞細胞定位：Secreted. Note=Released from the secretory granules upon mast cell activation.
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蛋白家族：Peptidase S1 family, Tryptase subfamily
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數據庫鏈接：
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