Human Tripartite motif-containing protein 72(TRIM72) ELISA kit

Human Tripartite motif-containing protein 72(TRIM72) ELISA kit

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中文名稱：

人三結構域包含蛋白72(TRIM72)酶聯(lián)免疫試劑盒
貨號：

CSB-EL024511HU
規(guī)格：

96T/48T
價格：

￥3600/￥2500
促銷：
其他：

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人三結構域包含蛋白72(TRIM72)酶聯(lián)免疫試劑盒（CSB-EL024511HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的TRIM72含量。TRIM72是一種三結構域蛋白，參與細胞膜修復和抗氧化功能，可能通過膜修復、抗氧化和線粒體功能修復等機制減緩神經(jīng)退行性疾病如肌萎縮側(cè)索硬化癥（ALS）的神經(jīng)元損害。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，適用于探究TRIM72在肌肉損傷修復、再生醫(yī)學或疾病模型（如心肌缺血、骨骼肌病變等）中的分子機制研究；可滿足細胞生物學、分子病理學等領域的基礎科研需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

LOC493829 ELISA Kit; Mg53 ELISA Kit; Mitsugumin-53 ELISA Kit; TRI72_HUMAN ELISA Kit; trim72 ELISA Kit; Tripartite motif containing 72 ELISA Kit; Tripartite motif containing protein 72 ELISA Kit; Tripartite motif-containing protein 72 ELISA Kit
縮寫：

TRIM72
Uniprot No.：

Q6ZMU5
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

25 pg/mL-1600 pg/mL
靈敏度：

6.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TRIM72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-98
1:2	Average %	92
	Range %	86-95
1:4	Average %	85
	Range %	80-89
1:8	Average %	92
	Range %	86-97

回收率：

The recovery of human TRIM72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	98-107
EDTA plasma (n=4)	95	90-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.508	2.310	2.409	2.284
800	1.442	1.345	1.394	1.269
400	0.779	0.816	0.798	0.673
200	0.463	0.446	0.455	0.330
100	0.322	0.317	0.320	0.195
50	0.239	0.231	0.235	0.110
25	0.198	0.192	0.195	0.070
0	0.126	0.124	0.125

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

The Relationship Between Serum MG53 Levels and the Presence of Metabolic Syndrome and Its Components B ANDA?, E ?ZGüN, M OKUR, AC YEKDE?,Medicina,2025
The Relationship Between Serum MG53 Levels and the Presence of Metabolic Syndrome and Its Components S Yan?k ?olak, B Anda?, E ?zgün, B Y?lmaz Bülbül,Medicina,2025
Trim72 is a major host factor protecting against lethal Candida albicans infection W Tan, J Liu, R Yu, P Zhao, Y Liu, Q Lu, K Wang,PLoS pathogens,2024

功能：

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
基因功能參考文獻：
1. serum TRIM72 may be a potential biomarker for the diagnosis and the prognosis of colon cancer. PMID: 29806630
2. MG53 is an effective biomarker of myocardial injury and dysfunction in murine hearts. However, MG53 is not expressed in human heart and therefore does not hold utility as a clinical biomarker of myocardial injury PMID: 26790476
3. MG53 is a facilitator of rapid injury repair, a mediator of cell migration, and a modulator of myofibroblast differentiation during wound healing PMID: 26306047
4. Zn(2+) interacts with MG53 in protection against injury to the cell membrane PMID: 25869134
5. MG53 induces FAK ubiquitination with the aid of UBE2H during skeletal myogenesis. PMID: 24344130
6. MG53/TRIM72 protein can be directly applied as a therapeutic agent to increase membrane repair capacity of many cell types. PMID: 23699904
7. data reveal NM-IIA as a key cytoskeleton motor protein that facilitates vesicle trafficking during MG53-mediated cell membrane repair PMID: 22253476
8. membrane-delimited interaction between MG53 and PTRF contributes to initiation of cell membrane repair PMID: 21343302
9. MG53, annexin A1, and dysferlin localize to the t-tubule network and show enriched labeling at longitudinal tubules of the t-system in overstretch PMID: 21412170
10. Crystal structure of PRY-SPRY domain of human TRIM72. PMID: 19967786
11. Observational study and meta-analysis of gene-disease association. (HuGE Navigator) PMID: 19129174
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亞細胞定位：

Cell membrane, sarcolemma. Cytoplasmic vesicle membrane.
蛋白家族：

TRIM/RBCC family
數(shù)據(jù)庫鏈接：

HGNC: 32671

OMIM: 613288

KEGG: hsa:493829

STRING: 9606.ENSP00000312675

UniGene: Hs.661859

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.