Human Thromboxane B2,TX-B2 ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人血栓素B2(TXB2)酶聯免疫試劑盒（CSB-E08046h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的TX-B2含量。TX-B2，即血栓素B2，是一種由血小板合成的脂質過氧化物，參與調節血管收縮和血小板聚集。其背景研究涉及血栓形成和心血管疾病。研究機制主要關注TX-B2在血栓形成過程中的作用，以及如何通過調節TX-B2水平來預防和治療相關疾病。試劑盒檢測范圍為9.38 pg/mL-600 pg/mL，該產品廣泛應用于心血管疾病機制研究、抗血小板藥物藥效評估、炎癥信號通路探索等科研領域，例如通過檢測阿司匹林干預后血小板釋放的 TXB2 水平變化，或分析動脈粥樣硬化模型動物組織中的血栓素代謝動態本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

TX-B2
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

9.38 pg/mL-600 pg/mL
靈敏度：

2.34 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TX-B2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
Range %	80-105
1:2	Average %	95
Range %	85-108
1:4	Average %	106
Range %	90-112
1:8	Average %	95
Range %	80-110

回收率：

The recovery of human TX-B2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	80-110
EDTA plasma (n=4)	98	90-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.