Human Thrombospondin type-1 domain-containing protein 4(THSD4) ELISA kit

Human Thrombospondin type-1 domain-containing protein 4(THSD4) ELISA kit

產(chǎn)品詳情

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品評(píng)價(jià)

相關(guān)產(chǎn)品

靶點(diǎn)詳情

Most popular with customers

產(chǎn)品中心

信號(hào)通路

技術(shù)信息

聯(lián)系信息

說明書下載

中文名稱：

人血小板反應(yīng)素1型結(jié)構(gòu)域蛋白4(THSD4)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL023518HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品描述：

人血小板反應(yīng)素1型結(jié)構(gòu)域蛋白4(THSD4)酶聯(lián)免疫試劑盒（CSB-EL023518HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的THSD4含量。THSD4是一種預(yù)測(cè)具有水解酶活性和細(xì)胞外基質(zhì)結(jié)構(gòu)成分的蛋白，可能在彈性纖維組件中起作用，位于含膠原蛋白的細(xì)胞外基質(zhì)和細(xì)胞外外泌體中。其研究機(jī)制涉及遺傳性血小板病，如巨血小板綜合征和血小板無力癥，以及其在止血、炎癥和免疫反應(yīng)中的作用。試劑盒檢測(cè)范圍為12.5 pg/mL-800 pg/mL，該產(chǎn)品適用于基礎(chǔ)研究中對(duì)THSD4表達(dá)水平的定量分析，如探究其在心血管疾病、纖維化病變或腫瘤進(jìn)展中的分子機(jī)制，亦可用于體外培養(yǎng)細(xì)胞模型中THSD4分泌水平的動(dòng)態(tài)監(jiān)測(cè)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

THSD4 ELISA Kit; UNQ9334/PRO34005 ELISA Kit; Thrombospondin type-1 domain-containing protein 4 ELISA Kit; A disintegrin and metalloproteinase with thrombospondin motifs-like protein 6 ELISA Kit; ADAMTS-like protein 6 ELISA Kit; ADAMTSL-6 ELISA Kit
縮寫：

THSD4
Uniprot No.：

Q6ZMP0
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human THSD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	88
	Range %	81-95
1:40	Average %	91
	Range %	86-97
1:80	Average %	97
	Range %	91-102
1:160	Average %	102
	Range %	97-107

回收率：

The recovery of human THSD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	95-110
EDTA plasma (n=4)	93	85-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.675	2.870	2.773	2.674
400	2.324	2.345	2.335	2.236
200	1.618	1.655	1.637	1.538
100	0.864	0.901	0.883	0.784
50	0.502	0.496	0.499	0.400
25	0.324	0.345	0.335	0.236
12.5	0.198	0.200	0.199	0.100
0	0.101	0.096	0.099

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Promotes FBN1 matrix assembly. Attenuates TGFB signaling, possibly by accelerating the sequestration of large latent complexes of TGFB or active TGFB by FBN1 microfibril assembly, thereby negatively regulating the expression of TGFB regulatory targets, such as POSTN.
基因功能參考文獻(xiàn)：
1. In breast cancer, some GATA3 effects shift from tumor suppressing to tumor promoting during tumorigenesis, with deregulation of three genes, BCL2, DACH1, THSD4, representing major GATA3-controlled processes in cancer progression. PMID: 25410484
2. ADAMTSL6beta has a role in fibrillin-1 microfibril formation PMID: 21880733
3. THSD4 polymorphism was not found to be associated with COPD risk PMID: 21965014
4. Variations may be important determinants of osteoporosis in Japanese women. PMID: 18488137
亞細(xì)胞定位：

Secreted, extracellular space, extracellular matrix.
數(shù)據(jù)庫鏈接：

HGNC: 25835

OMIM: 614476

KEGG: hsa:79875

STRING: 9606.ENSP00000261862

UniGene: Hs.387057

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.