Human Thiosulfate sulfurtransferase(TST) ELISA kit

Human Thiosulfate sulfurtransferase(TST) ELISA kit

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中文名稱：

人硫代硫酸硫轉(zhuǎn)移酶(TST)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL025197HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人硫代硫酸硫轉(zhuǎn)移酶(TST)酶聯(lián)免疫試劑盒（CSB-EL025197HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的TST含量。人硫代硫酸硫轉(zhuǎn)移酶（TST）是一種線粒體酶，主要功能是催化硫代硫酸鹽和氰根離子轉(zhuǎn)化為亞硫酸鹽和硫氰酸鹽，參與代謝和解毒過程。它在氧化還原反應(yīng)中起關(guān)鍵作用，可能影響蛋白質(zhì)穩(wěn)定性和折疊，并在翻譯過程中對(duì)tRNA核苷的轉(zhuǎn)硫化起重要作用。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于科研場(chǎng)景下探究TST在硫代謝通路中的功能機(jī)制、氧化應(yīng)激相關(guān)疾病模型中的表達(dá)動(dòng)態(tài)，或用于藥物研發(fā)中化合物對(duì)TST活性影響的評(píng)估本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

GlpE ELISA Kit; RDS ELISA Kit; Rhodanese ELISA Kit; Thiosulfate sulfurtransferase ELISA Kit; Thiosulfate sulfurtransferase GlpE ELISA Kit; THTR_HUMAN ELISA Kit; TST ELISA Kit
縮寫：

TST
Uniprot No.：

Q16762
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	95
	Range %	91-105
1:4	Average %	100
	Range %	92-110
1:8	Average %	92
	Range %	86-98

回收率：

The recovery of human TST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	95	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.504	2.511	2.508	2.352
500	2.131	2.052	2.092	1.936
250	1.415	1.333	1.374	1.218
125	0.759	0.744	0.752	0.596
62.5	0.541	0.523	0.532	0.376
31.2	0.337	0.323	0.330	0.174
15.6	0.251	0.244	0.248	0.092
0	0.159	0.153	0.156

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity. Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.
基因功能參考文獻(xiàn)：
1. TST mRNA expression in adipose tissue correlated positively with insulin sensitivity in adipose tissue and negatively with fat mass. PMID: 27270587
2. show that polymorphic variations that are distant from the active site differentially modulate the sulfurtransferase activity of human rhodanese to cyanide versus sulfite PMID: 26269602
3. Loss of Thiosulfate Sulfurtransferase is associated with decreased mucosal sulfide detoxification capacity in patients with Crohn's disease PMID: 23399736
4. silencing of the rhodanese gene caused not only a proportional decrease of 5 S rRNA import but also a general inhibition of mitochondrial translation, indicating the functional importance of the imported 5 S rRNA inside the organelle. PMID: 20663881
5. Proteomics was used to study colonic epithelial aging, for differential proteins in the human normal colonic epithelial tissues from young and old people. Rack1, EF-Tu and Rhodanese, three validated differential proteins, were further investigated. PMID: 20099848
6. A partially gluconoylated N-terminus of the His6-tagged molybdenum cofactor synthesis 3 (MOCS3)-rhodanese-like domain is identified which results in a heterogeneity of the protein but does not influence sulfurtransferase activity. PMID: 15910006
7. The USP8 recognition domain of NRDP1 has a novel protein fold that interacts with a conserved peptide loop of the rhodanese domain. PMID: 17035239
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亞細(xì)胞定位：

Mitochondrion matrix.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 12388

OMIM: 180370

KEGG: hsa:7263

STRING: 9606.ENSP00000249042

UniGene: Hs.474783

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.