Human Spondin-2 ELISA Kit

Human Spondin-2 ELISA Kit

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中文名稱：

人Spondin-2酶聯免疫試劑盒
貨號：

CSB-E14967h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人Spondin-2酶聯免疫試劑盒（CSB-E14967h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、腦脊液（CSF）、組織培養上清液樣本中的SPON2含量。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，該產品適用于基礎科研領域，如探究Spondin-2在腫瘤發生、神經退行性疾病或組織修復中的分子機制，也可用于體外細胞模型中蛋白分泌水平的動態監測，為疾病相關生物標志物研究及信號通路調控分析提供高效工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Differentially expressed in cancerous and non-cancerous lung cells 1 ELISA Kit; DIL 1 ELISA Kit; DIL-1 ELISA Kit; DIL1 ELISA Kit; DKFZp686G21139 ELISA Kit; FLJ16313 ELISA Kit; FLJ22401 ELISA Kit; FLJ34460 ELISA Kit; M spondin ELISA Kit; Mindin ELISA Kit; ORF Names: ELISA Kit; SPON 2 ELISA Kit; Spon2 ELISA Kit; SPON2_HUMAN ELISA Kit; Spondin 2 ELISA Kit; Spondin 2 extracellular matrix protein ELISA Kit; Spondin-2 ELISA Kit
縮寫：

SPON2
Uniprot No.：

Q9BUD6
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cerebrospinal fluid (CSF), cell culture supernates
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	97
	Range %	91-100
1:4	Average %	97
	Range %	92-104
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human spondin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	1.825	1.897	1.861	1.744
100	1.414	1.435	1.425	1.308
50	0.996	0.981	0.989	0.872
25	0.692	0.683	0.688	0.571
12.5	0.423	0.458	0.441	0.324
6.25	0.295	0.280	0.288	0.171
3.12	0.206	0.216	0.211	0.094
0	0.116	0.118	0.117

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Nové biomarkery jako parametry pro predikci signifikantního karcinomu prostaty v biopsii a recidivy onemocnění po radikální lé?bě J ?ezá?,Univerzita Karlova, 2. léka?ská fakulta,2024
Diagnostic benefits of mindin as a prostate cancer biomarker Lenka Hanouskova, et al,JOURNAL OF MEDICAL BIOCHEMISTRY,2019

功能：

Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens. Binds bacterial lipopolysaccharide (LPS).
基因功能參考文獻：
1. Egr-1 regulates mindin expression by directly binding to the mindin promoter; mindin suppresses colon cancer progression by blocking angiogenesis. PMID: 28991232
2. Spondin-2 overexpression contributes to tumor aggressiveness and prognosis in gastric cancer. PMID: 28060752
3. SPON2 is a transcriptional target of the metastasis gene MACC1. SPON2 induces cell motility in vitro and CRC metastasis in mice. In patients, SPON2 serves as prognostic indicator for CRC metastasis and survival, and might represent a promising target for therapeutic approaches. PMID: 26686083
4. Data suggest that spondin-2 could be an independent diagnostic and prognostic biomarker of colon cancer. PMID: 25945835
5. Mindin protects against vascular hyperplasia by suppression of abnormal VSMC proliferation, migration and phenotypic switching in an AKT-dependent manner. PMID: 25751394
6. Using tissue microarray by immunohistostaining, we found SPON2 to be over-expressed in prostate cancer (PCa). SPON2 staining was more intense in Gleason score sum 7-8 and in PCa patients with metastasis. PMID: 22615945
7. mindin serves as a novel mediator that protects against cardiac hypertrophy and the transition to heart failure by blocking AKT/GSK3beta and TGF-beta1-Smad signalling. PMID: 21632881
8. High Mindin is associated with diabetic nephropathy in type 2 diabetes. PMID: 21098016
9. Spondin 2, which is regulated by T(3), has an important role in cell invasion, cell migration, and hepatoma tumor progression. PMID: 19903741
10. The structure of the F-spondin domain of mindin was determined at 1.8-A resolution. PMID: 19153605
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亞細胞定位：

Secreted, extracellular space, extracellular matrix.
組織特異性：

Expressed in normal lung tissue but not in lung carcinoma cell lines.
數據庫鏈接：

HGNC: 11253

OMIM: 605918

KEGG: hsa:10417

STRING: 9606.ENSP00000290902

UniGene: Hs.302963

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.