Human Small Dense Low Density Lipoprotein(sLDL)ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人小而密低密度脂蛋白（sLDL）酶聯(lián)免疫試劑盒（CSB-E13350h）為競爭法ELISA試劑盒，定量檢測血清、血漿、唾液、尿液、組織勻漿樣本中的sLDL含量。sLDL（小而密低密度脂蛋白）是一種特殊的脂蛋白，其顆粒較小、密度較高，易被氧化且更容易進(jìn)入血管壁，在動脈粥樣硬化等心血管疾病的發(fā)生發(fā)展中起重要作用。目前研究多聚焦于其致動脈粥樣硬化的機(jī)制以及降低 sLDL 水平的干預(yù)措施。試劑盒檢測范圍為0.312 nmol/mL-20 nmol/mL，支持科研人員高效開展心血管疾病發(fā)病機(jī)制研究、降脂藥物作用效果評估、代謝綜合征相關(guān)模型構(gòu)建等實(shí)驗。適用于體外實(shí)驗環(huán)境下的基礎(chǔ)研究、藥理學(xué)研究及生物標(biāo)志物探索等科研場景。為縱向追蹤研究或大規(guī)模樣本分析提供便利。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

sLDL
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, saliva, urine, tissue homogenates
檢測范圍：

0.312 nmol/mL-20 nmol/mL
靈敏度：

0.078 nmol/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human sLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	92-103
1:200	Average %	92
	Range %	86-96
1:400	Average %	104
	Range %	97-107
1:800	Average %	84
	Range %	80-88

回收率：

The recovery of human sLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-93
EDTA plasma (n=4)	92	88-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.