Human Signal Peptide, CUB and EGF-like Domain-containing Protein 1(SCUBE1)ELISA Kit

Human Signal Peptide, CUB and EGF-like Domain-containing Protein 1(SCUBE1)ELISA Kit

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中文名稱：

人信號(hào)肽,CUB和EGF樣結(jié)構(gòu)域蛋白1(SCUBE1)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E15005h
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
促銷：
其他：

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產(chǎn)品描述：

人信號(hào)肽,CUB和EGF樣結(jié)構(gòu)域蛋白1(SCUBE1)酶聯(lián)免疫試劑盒（CSB-E15005h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SCUBE1含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于基礎(chǔ)科研中探索SCUBE1在疾病模型中的表達(dá)動(dòng)態(tài)、信號(hào)通路關(guān)聯(lián)性分析，或作為分子標(biāo)志物在細(xì)胞實(shí)驗(yàn)、動(dòng)物模型及臨床前樣本中的定量評估，為研究血管生成、組織修復(fù)及病理機(jī)制提供可靠工具。滿足分子生物學(xué)及轉(zhuǎn)化醫(yī)學(xué)研究的精準(zhǔn)檢測需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

SCUB1_HUMAN ELISA Kit; SCUBE 1 ELISA Kit; SCUBE1 ELISA Kit; Signal peptide, CUB and EGF-like domain-containing protein 1 ELISA Kit; Signal peptide, CUB domain, EGF like 1 ELISA Kit
縮寫：

SCUBE1
Uniprot No.：

Q8IWY4
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SCUBE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	89
	Range %	82-94
1:10	Average %	96
	Range %	91-105
1:20	Average %	99
	Range %	95-108
1:40	Average %	94
	Range %	86-102

回收率：

The recovery of human SCUBE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	106	99-110
EDTA plasma (n=4)	92	84-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.670	2.592	2.631	2.502
20	2.271	2.173	2.222	2.093
10	1.631	1.615	1.623	1.494
5	0.940	0.925	0.933	0.804
2.5	0.512	0.502	0.507	0.378
1.25	0.357	0.347	0.352	0.223
0.625	0.226	0.219	0.223	0.094
0	0.130	0.127	0.129

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

The assessment of the relationship between the development of cardiovascular diseases and SCUBE-1 level in individuals exposed to loud noise H Fedai, K Toprak, M Cetin, AN Elmas, V Tosun,Medicine,2024
Biomarkers In Prediction of Acute Mesenteric Ischaemia: a prospective multicentre study (BIPAMI study): a study protocol K Tamme,BMJ open,2024
SCUBE1 promotes pulmonary artery smooth muscle cell proliferation and migration in acute pulmonary embolism by modulating BMP7 L Jia,PeerJ,2024
The diagnostic potential of plasma SCUBE‐1 concentration for pulmonary embolism: A pilot study L Xiao,The clinical respiratory journal,2023
CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma J Zhao,Journal of translational medicine,2022
Can serum SCUBE1 levels be useful in the diagnosis of bladder cancer? A MENTE?E,Journal of Experimental and Clinical Medicine,2022
Pericardial SCUBE1 levels may help predict postoperative results in patients operated on for coronary artery bypass graft surgery T ?zkara,CARDIOVASCULAR JOURNAL OF AFRICA,2021
Diagnostic value of signal peptide-Complement C1r/C1s, Uegf, and Bmp1-epidermal growth factor domain-containing protein 1 on serum and tissue samples in non-small cell lung cancer Selcuk Gurz.et al,/,2018
The Diagnostic Value of Scube1 in Patients Presenting to the Emergency Department Due to Angina Pectoris Aynur SAHIN.et al,/,2018
The predictive value of signal peptide-CUB-EGF domain-containing protein1 (SCUBE1) in terms of the duration of peripheral ischemia and exposure to reperfusion Ozgur Sogut.et al,Biomed Res Int.,2016
The Diagnostic Value of Plasma SCUBE1, a Novel Biomarker of Platelet Activation, in Testicular Torsion: A Randomized, Controlled, Experimental Study Turedi S.et al,Urology,2015
Increased serum concentrations of signal peptide-Cub-Egf domain-containing protein-1 in patients with aneurysmal subarachnoid hemorrhage. Ding YS.et al,Clin Chim Acta.,2016
The diagnostic role of signal peptide-C1r/C1s, Uegf, and Bmp1-epidermal growth factor domain-containing protein 1 in non-ST-elevation acute coronary syndrome. Sonmez E.et al,Am J Emerg Med.,2015
The role of hypoxia at primary dysmenorrhea, utilizing a novel hypoxia marker--SCUBE1. Tekin YB.et al,J Pediatr Adolesc Gynecol.,2015
Elevated Serum Levels of SCUBE1, a Marker for Coagulation, in Patients with Breast Cancer Topcu TO.et al,Tohoku J Exp Med.,2015
Role of signal peptide-Cub-Egf domain-containing protein-1 in serum as a predictive biomarker of outcome after severe traumatic brain injury Chen QH.et al,Clin Chim Acta.,2016
Evaluation of SCUBE-1 and sCD40L biomarkers in patients with hypothyroidism due to Hashimoto's thyroiditis: a single-blind, controlled clinical study Bilir B.et al,Eur Rev Med Pharmacol Sci.,2016
The diagnostic value of SCUBE1 levels in acute ischemic stroke Mücahit Günaydin et al,Türk Biyokimya Dergisi,2014
Signal peptide-UB-GF domain-containing protein 1 (SCUBE1) levels in patients with overt and subclinical hyperthyroidism: effects of treatment Erem C. et al,Clin Endocrinol (Oxf),2015
Signal peptide-CUB-EGF domain-containing protein 1 (SCUBE1) level in hemodialysis patients and parameters affecting that level /,Clinical Biochemistry,2012
The diagnostic and prognostic significance of SCUBE1 levels in Crimean-Congo hemorrhagic fever Mentese A et al,Int J Infect Dis.,2013

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功能：

Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.
基因功能參考文獻(xiàn)：
1. The results of this pioneering study indicate that SCUBE protein family appears to have a probable role in the pathogenesis and angiogenesis development in psoriasis and SCUBE 1 and 3 may be novel markers of angiogenesis in psoriasis. PMID: 28238185
2. enhanced SCUBE1 concentrations are correlated with increasing severity and poor outcomes of aneurysmal subarachnoid hemorrhage patients, indicating SCUBE1 might have the potential to identify aneurysmal subarachnoid hemorrhage patients at risk of poor prognosis PMID: 27265525
3. Increased serum SCUBE1 concentrations have close relation to increasing severity and poor prognosis of intracerebral hemorrhage PMID: 27496080
4. SCUBE1 might be a novel prognostic biomarker after traumatic brain injury PMID: 26940951
5. describe novel mechanisms in targeting S1 to the plasma membrane and demonstrate that N-glycans are required for S1 functions during primitive haematopoiesis in zebrafish. PMID: 26699903
6. SCUBE1 may indicate hypercoagulability in patients with BC and thus help identify patients at greater risk of thrombosisSCUBE1 may indicate hypercoagulability in patients with breast cancer and thus help identify patients at greater risk of thrombosis PMID: 26438214
7. SCUBE1 levels are a new biomarker that can be used in the differential diagnosis and monitoring of patients hospitalized with suspected CCHF. PMID: 23632301
8. Primary prostatic cancer-associated fibroblasts (CAFs) were isolated and the expression of CAF markers, Dlk1 and SCUBE1 transcripts were characterised. PMID: 23136397
9. SCUBE 1 levels in patients with gastric cancer were found higher compared to healthy subjects. PMID: 23220851
10. SCUBE1 level, a potential acute ischemia marker, is elevated in hemodialysis patients with no clinical ischemic event. PMID: 22874483
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亞細(xì)胞定位：

Secreted. Cell membrane; Peripheral membrane protein.
組織特異性：

Detected in endothelial cells. Highly expressed in platelets. Stored in platelet alpha granules, and transferred to the cell surface upon activation and aggregation. A smaller form, probably produced by limited proteolysis, after being released from the s
數(shù)據(jù)庫鏈接：

HGNC: 13441

OMIM: 611746

KEGG: hsa:80274

STRING: 9606.ENSP00000354080

UniGene: Hs.133995

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.