Human Sialic acid-binding Ig-like lectin 9(SIGLEC9) ELISA kit
-
中文名稱：人唾液酸結合Ig樣凝集素9(SIGLEC9)酶聯免疫試劑盒
-
貨號：CSB-EL021303HU
-
規格：96T/48T
-
價格：￥3600/￥2500
-
促銷：
-
其他：
產品詳情
-
產品描述：人唾液酸結合Ig樣凝集素9(SIGLEC9)酶聯免疫試劑盒（CSB-EL021303HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿，組織勻漿.樣本中的SIGLEC9含量。SIGLEC9是唾液酸結合免疫球蛋白樣凝集素家族成員，廣泛分布于免疫細胞表面。它能識別細胞表面糖基配體，參與免疫信號傳導，調控免疫細胞活性和功能。當前圍繞其展開多項研究，旨在明晰機制，為免疫相關疾病治療提供潛在靶點。試劑盒檢測范圍為125 pg/ml-8000 pg/ml，適用于科研場景中探究SIGLEC9在免疫應答、疾病模型或病理狀態下的動態變化，例如通過檢測不同處理條件下細胞培養上清、實驗動物體液或疾病相關組織樣本中的蛋白濃度，為免疫調控機制、生物標志物篩選等基礎研究提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
-
別名：CD329 ELISA Kit; CDw329 ELISA Kit; FOAP 9 ELISA Kit; OB binding protein like ELISA Kit; OBBP LIKE ELISA Kit; Protein FOAP 9 ELISA Kit; Protein FOAP-9 ELISA Kit; sialic acid binding Ig like lectin 9 ELISA Kit; Sialic acid-binding Ig-like lectin 9 ELISA Kit; Sialic acid-binding immunoglobulin like lectin 9 ELISA Kit; SIGL9_HUMAN ELISA Kit; Siglec 9 ELISA Kit; Siglec-9 ELISA Kit; SIGLEC9 ELISA Kit
-
縮寫：
-
Uniprot No.：
-
種屬：Homo sapiens (Human)
-
樣本類型：serum,plasma,tissue homogenates.
-
檢測范圍：125 pg/ml-8000 pg/ml
-
靈敏度：31.25 pg/ml
-
反應時間：1-5h
-
樣本體積：50-100ul
-
檢測波長：450 nm
-
研究領域：Immunology
-
測定原理：quantitative
-
測定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
-
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human SIGLEC9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
95
Range %
88-102
1:2
Average %
87
Range %
83-92
1:4
Average %
92
Range %
88-97
1:8
Average %
89
Range %
85-95
-
回收率：
The recovery of human SIGLEC9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
90
85-96
EDTA plasma (n=4)
96
90-102
-
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
8000
2.169
2.234
2.202
2.068
4000
1.378
1.297
1.338
1.205
2000
0.831
0.828
0.829
0.696
1000
0.474
0.489
0.481
0.348
500
0.383
0.361
0.372
0.239
250
0.250
0.253
0.251
0.118
125
0.186
0.184
0.185
0.052
0
0.137
0.129
0.133
-
數據處理：
-
貨期：3-5 working days
產品評價
相關產品
靶點詳情
-
功能：Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
-
基因功能參考文獻：
- The SIGLEC9 rs2075803 G/rs2258983 A haplotype, which corresponds to a Siglec-9 variant that is less effective at suppressing inflammatory response, may be a risk factor for the development of emphysema. PMID: 27878892
- Glycophorin A, the most abundant sialoglycoprotein on erythrocytes, engaged neutrophil Siglec-9, a sialic acid-recognizing receptor known to dampen innate immune cell activation. These studies demonstrate a previously unsuspected role for erythrocytes in suppressing neutrophils ex vivo and in vitro and help explain why neutrophils become easily activated after separation from whole blood. PMID: 28416510
- Data suggest that sialic acid-binding Ig-like lectin-9 (Siglec-9)may serve as a potential diagnostic and therapeutic target for rheumatoid arthritis (RA). PMID: 28273363
- The mechanism of the Siglec-9 and AOC3 interaction is mediated both by protein-sugar interactions via the V domain and by the protein-protein interactions via the C22 Siglec9 domain. PMID: 27893774
- This work defines a critical role for aberrantly glycosylated MUC1 and identifies an activating pathway that follows engagement of Siglec-9. PMID: 27595232
- Constitutively expressed Siglec-9 inhibits LPS-induced CCR7, but enhances IL-4-induced CD200R expression in human macrophages. PMID: 26923638
- Ligands for Siglec-8 and Siglec-9 may regulate the function of eosinophils, mast cells, neutrophils, and other cells in sinus mucosa. PMID: 26694037
- Inflammation results in up-regulation of immuneinhibitory Siglec-8 and Siglec-9 sialoglycan ligands on human airways. PMID: 25747723
- a polymorphism that reduced Siglec-9 binding to carcinomas was associated with improved early survival in non-small-cell lung cancer patients PMID: 25225409
- Dasatinib enhances migration of monocyte-derived dendritic cells by reducing phosphorylation of inhibitory immune receptors Siglec-9 and Siglec-3. PMID: 24882272
- Protein degradation of focal adhesion kinase and related molecules was induced by Siglec-9 binding to its counterreceptors via sialylglycoconjugates, leading to the modulation of adhesion kinetics of cancer cells. PMID: 24145038
- Siglec-9 expressed on immune cells may play a role as a potential counterreceptor for MUC1 and that this signaling may be another MUC1-mediated pathway and function in parallel with a growth factor-dependent pathway. PMID: 24045940
- The Siglec-9 peptide binding to the enzymatic groove of VAP-1 can be used for imaging conditions, such as inflammation and cancer. PMID: 21821708
- Siglec-9 A391C was the only polymorphism related to TCR-mediated signaling in human Siglec-9, resulting in less inhibition compared to the wild type. PMID: 20733319
- These results suggest that Siglec-9 expressed on DCs is involved in immunoregulation through ligation with mucins in an epithelial cancer patient. PMID: 20971061
- MUC16-Siglec-9 binding likely mediates inhibition of anti-tumor immune responses. PMID: 20497550
- Septic shock patients exhibit different ex vivo death responses of blood neutrophils after Siglec-9 ligation early in shock. PMID: 19295491
- Binding studies on recombinant human Siglec-9 show recognition of both Neu5Ac and Neu5Gc; in striking contrast, chimpanzee and gorilla Siglec-9 strongly prefer binding Neu5Gc. PMID: 14693915
- Siglec-9 can inhibit Fc epsilon receptor I-mediated serotonin release from rat basophilic leukemia cells and recruit the tyrosine phosphatases SHP-1 and SHP-2. PMID: 15557178
- data suggest that apoptotic (ROS- and caspase-dependent) and nonapoptotic (ROS-dependent) death pathways are initiated in neutrophils via Siglec-9 PMID: 15827126
- Siglecs-9 did not interact with sulfate derivatives of LacNAc and sulfated oligosaccharides containing sialic acid. PMID: 16732727
- The Siglec-9 provides not only a useful marker for certain subsets of AML, but also a potential therapeutic target. PMID: 16828866
- These results demonstrate that Siglec-9 enhances the production of the anti-inflammatory cytokine IL-10 in macrophages. PMID: 18325328
- A review of the cytotoxic effects of natural anti-Siglec9 autoantibodies on both neutrophils and eosinophils. PMID: 18558361
- Increased bacterial survival are also facilitated by group b Streptococcus sialylated capsular polysaccharide interactions with Siglec-9. PMID: 19196661
- Data suggest that age-specific interactions between Siglec-9 and SHP-1 may influence the altered inflammatory responsiveness and longevity of neonatal polymorphonuclear neutrophils. PMID: 19542910
顯示更多
收起更多
-
亞細胞定位：Membrane; Single-pass type I membrane protein.
-
蛋白家族：Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
-
組織特異性：Expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils). Found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adre
-
數據庫鏈接：
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
